Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial, Homo sapiens (Human), E.coli, C-terminal 6xHis-tagged, 26.1kDa, purity >95% as determined by SDS-PAGE.

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial — DNA helicase that plays an important role in DNA replication, transcription and repair. Purity >95%.

SKU: BCRECP-001529 Category:
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Product Specifications

Product SkuBCRECP-001529
Product DescriptionRecombinant Human ATP-dependent DNA helicase Q5 (RECQL5) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 39-213aa. [Accession | O94762].
Uniprot No.O94762
Gene NamesRECQL5
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region39-213aa
SpeciesHomo sapiens (Human)
Tag InfoC-terminal 6xHis-tagged
Molecular weight26.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

DNA helicase that plays an important role in DNA replication, transcription and repair. Probably unwinds DNA in a 3'-5' direction (Probable). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability.

Biological Context

Subcellular Location: Cytoplasm
Tissue Specificity: Ubiquitous

Product Specifications

Recombinant Human ATP-dependent DNA helicase Q5 (RECQL5), partial is a recombinant protein from Homo sapiens (Human), expressed in E.coli, covering amino acids 39-213aa, with C-terminal 6xHis-tagged tag, molecular weight 26.1kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

DNA helicase that plays an important role in DNA replication, transcription and repair (PubMed:20643585, PubMed:22973052, PubMed:28100692). Probably unwinds DNA in a 3'-5' direction (Probable) (PubMed:28100692). Binds to the RNA polymerase II subunit POLR2A during transcription elongation and suppresses transcription-associated genomic instability (PubMed:20231364). Also associates with POLR1A and enforces the stability of ribosomal DNA arrays (PubMed:27502483). Plays an important role in mitotic chromosome separation after cross-over events and cell cycle progress (PubMed:22013166). Mechanistically, removes RAD51 filaments protecting stalled replication forks at common fragile sites and stimulates MUS81-EME1 endonuclease leading to mitotic DNA synthesis (PubMed:28575661). Required for efficient DNA repair, including repair of inter-strand cross-links (PubMed:23715498). Stimulates DNA decatenation mediated by TOP2A. Prevents sister chromatid exchange and homologous recombination. A core helicase fragment (residues 11-609) binds preferentially to splayed duplex, looped and ssDNA (PubMed:28100692)

Subcellular Location

Cytoplasm

Tissue Specificity

Ubiquitous

Subunit

Monomer. Interacts with TOP2A, TOP3A and TOP3B (PubMed:10710432, PubMed:20231364). Isoform beta interacts with RNA polymerase II subunit POLR2A (PubMed:20231364, PubMed:20348101, PubMed:23748380). Identified in a complex with the RNA polymerase II core bound to DNA (PubMed:20231364, PubMed:20348101). Interacts (via C-terminus) with POLR1A (PubMed:27502483). Isoform beta interacts with RAD51 (PubMed:20231364, PubMed:20348101). Interacts with WRN; this interaction stimulates WRN helicase activity on DNA fork duplexes (PubMed:23180761). Interacts with MUS1; this interaction promotes MUS81-dependent mitotic DNA synthesis (PubMed:28575661)

Gene: RECQL5  |  Organism: Homo sapiens  |  Synonyms: DNA 3'-5' helicase RecQ5; DNA helicase, RecQ-like type 5; RecQ protein-like 5
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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