Recombinant Escherichia coli Microcin J25 (mcjA), Escherichia coli, E.coli, N-terminal 6xHis-KSI-tagged, 17.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli Microcin J25 (mcjA)

Recombinant Escherichia coli Microcin J25 (mcjA) — Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). Purity >90%.

SKU: BCRECP-003367 Category:
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Product Specifications

Product SkuBCRECP-003367
Product DescriptionRecombinant Escherichia coli Microcin J25 (mcjA) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 38-58aa. [Accession | Q9X2V7].
Uniprot No.Q9X2V7
Gene NamesmcjA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region38-58aa
SpeciesEscherichia coli
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight17.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP). Inhibits transcription by binding deep within RNAP secondary channel, where it sterically blocks the folding of the trigger loop, which is essential for efficient catalysis. In addition, it also seems to restrict access of nucleotide substrates to the catalytic center, and shows a partially competitive mode of inhibition with them.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Escherichia coli Microcin J25 (mcjA) is a recombinant protein from Escherichia coli, expressed in E.coli, covering amino acids 38-58aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 17.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Peptide antibiotic that functions through inhibition of the bacterial DNA-dependent RNA polymerase (RNAP) (PubMed:11443089, PubMed:12401787). Inhibits transcription by binding deep within RNAP secondary channel, where it sterically blocks the folding of the trigger loop, which is essential for efficient catalysis (PubMed:15200952, PubMed:30626643). In addition, it also seems to restrict access of nucleotide substrates to the catalytic center, and shows a partially competitive mode of inhibition with them (PubMed:15200952, PubMed:30626643). Exhibits potent bacteriocidal activity against a range of Enterobacteriaceae, including several pathogenic E.coli, Salmonella and Shigella strains (PubMed:1429464). Also acts on the cytoplasmic membrane of Salmonella newport, producing alteration of membrane permeability and disruption of the subsequent gradient dissipation, which inhibits several processes essential for cell viability, such as oxygen consumption (PubMed:11731133). Induces bacterial filamentation in susceptible cells in a non-SOS-dependent way, but this phenotype may result from impaired transcription of genes coding for cell division proteins (PubMed:1429464)

Subcellular Location

Secreted

Gene: mcjA  |  Organism: Escherichia coli  |  Synonyms: Class II lasso peptide; Ribosomally synthesized and post-translationally modified peptide
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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