Recombinant Middle East respiratory syndrome-related coronavirus Orf1ab, partial, Middle East respiratory syndrome-related coronavirus, E.coli, Tag-Free, 106.9kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Middle East respiratory syndrome-related coronavirus Orf1ab, partial

Recombinant Middle East respiratory syndrome-related coronavirus Orf1ab, partial — recombinant protein from Middle East respiratory syndrome-related coronavirus, expressed in E.coli. Purity >90%.

SKU: BCRECP-003530 Categories: , , ,
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Product Specifications

Product SkuBCRECP-003530
Product DescriptionRecombinant Middle East respiratory syndrome-related coronavirus Orf1ab, partial Protein is expressed from E.coli with Tag-Free. It contains 4378-5310aa. [Accession | A0A0G3U5R1].
Uniprot No.A0A0G3U5R1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region4378-5310aa
SpeciesMiddle East respiratory syndrome-related coronavirus
Tag InfoTag-Free
Molecular weight106.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.

Product Specifications

Recombinant Middle East respiratory syndrome-related coronavirus Orf1ab, partial — recombinant protein from Middle East respiratory syndrome-related coronavirus, expressed in E.coli. Purity >90%. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products

Subcellular Location

Host cytoplasm, host perinuclear region; Host endoplasmic reticulum-Golgi intermediate compartment; Host membrane (Multi-pass membrane protein)

Subunit

Monomer. Homodimer. Only the homodimer shows catalytic activity

Organism: Middle East respiratory syndrome-related coronavirus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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