Recombinant Clostridium butyricum Botulinum neurotoxin type E, partial, Clostridium butyricum, E.coli, N-terminal 6xHis-SUMO-tagged, 63.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Clostridium butyricum Botulinum neurotoxin type E, partial

Recombinant Clostridium butyricum Botulinum neurotoxin type E, partial — Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Purity >90%.

SKU: BCRECP-003615 Category:
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Product Specifications

Product SkuBCRECP-003615
Product DescriptionRecombinant Clostridium butyricum Botulinum neurotoxin type E, partial Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 2-422aa. [Accession | P30995].
Uniprot No.P30995
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-422aa
SpeciesClostridium butyricum
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight63.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the cell surface.

Biological Context

Subcellular Location: Secreted; Host synapse, host presynaptic cell membrane; Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Clostridium butyricum Botulinum neurotoxin type E, partial is a recombinant protein from Clostridium butyricum, expressed in E.coli, covering amino acids 2-422aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 63.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD). The RBD is responsible for the adherence of the toxin to the cell surface. It simultaneously recognizes 2 coreceptors; host polysialated gangliosides and the receptor proteins SV2A and SV2B in close proximity on host synaptic vesicles. Interaction with SV2 proteins requires SV2 glycosylation. The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and protect toxin prior to translocation (By similarity). The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol (By similarity). Binds ganglioside GD1a in vitro (PubMed:21849494)

Subcellular Location

Secreted; Host synapse, host presynaptic cell membrane; Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane (Multi-pass membrane protein)

Subunit

Heterodimer; disulfide-linked heterodimer of a light chain (LC) and a heavy chain (HC). The LC has the proteolytic/pharmacological activity, while the N- and C-terminal of the HC mediate channel formation and toxin binding, respectively. Interacts with host synaptic vesicle glycoproteins SV2A and SV2B which probably serve as coreceptors

Organism: Clostridium butyricum  |  Synonyms: Bontoxilysin-E

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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