Recombinant Influenza A virus Neuraminidase (NA), partial, Influenza A virus (strain A/Niigata/137/1996 H3N2), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 56.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Influenza A virus Neuraminidase (NA), partial

Recombinant Influenza A virus Neuraminidase (NA), partial — Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Purity >90%.

SKU: BCRECP-003838 Categories: , ,
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Product Specifications

Product SkuBCRECP-003838
Product DescriptionRecombinant Influenza A virus Neuraminidase (NA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 31-469aa. [Accession | O91745].
Uniprot No.O91745
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region31-469aa
SpeciesInfluenza A virus (strain A/Niigata/137/1996 H3N2)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight56.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface.

Biological Context

Subcellular Location: Virion membrane; Host apical cell membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Influenza A virus Neuraminidase (NA), partial is a recombinant protein from Influenza A virus (strain A/Niigata/137/1996 H3N2), expressed in E.coli, covering amino acids 31-469aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 56.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates. Cleaves off the terminal sialic acids on the glycosylated HA during virus budding to facilitate virus release. Additionally helps virus spread through the circulation by further removing sialic acids from the cell surface. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell. Otherwise, infection would be limited to one round of replication. Described as a receptor-destroying enzyme because it cleaves a terminal sialic acid from the cellular receptors. May facilitate viral invasion of the upper airways by cleaving the sialic acid moieties on the mucin of the airway epithelial cells. Likely to plays a role in the budding process through its association with lipid rafts during intracellular transport. May additionally display a raft-association independent effect on budding. Plays a role in the determination of host range restriction on replication and virulence. Sialidase activity in late endosome/lysosome traffic seems to enhance virus replication

Subcellular Location

Virion membrane; Host apical cell membrane (Single-pass type II membrane protein)

Subunit

Homotetramer

Gene: NA  |  Organism: Influenza A virus (strain A/Niigata/137/1996 H3N2)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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