Recombinant Mouse NELL2-interacting cell ontogeny regulator 1 (Nicol1), Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 13.8kDa, purity >95% as determined by SDS-PAGE.

Recombinant Mouse NELL2-interacting cell ontogeny regulator 1 (Nicol1)

Recombinant Mouse NELL2-interacting cell ontogeny regulator 1 (Nicol1) — mRNA-binding protein which interacts with a range of target mRNAs including SERPINE1, ACTA2, CCN2 and COL4A1 and may promote extracellular matrix production. Purity >95%.

SKU: BCRECP-003873 Category:
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Product Specifications

Product SkuBCRECP-003873
Product DescriptionRecombinant Mouse NELL2-interacting cell ontogeny regulator 1 (Nicol1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 29-90aa. [Accession | Q3UR78].
Uniprot No.Q3UR78
Gene NamesNicol1
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region29-90aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight13.8kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

mRNA-binding protein which interacts with a range of target mRNAs including SERPINE1, ACTA2, CCN2 and COL4A1 and may promote extracellular matrix production. Binds to the 3'-UTR of SERPINE1 mRNA and stabilizes the mRNA, possibly by competing for binding with SERBP1 and preventing SERBP1-mediated mRNA degradation. Also binds to the 3'-UTR of ACTA2.

Biological Context

Subcellular Location: Secreted; Cytoplasm, perinuclear region
Tissue Specificity: Expression is enriched in both male and female reproductive organs, including the testis, epididymis, seminal vesicles, coagulating glands, ovary and uterus, and in various non-reproductive organs such as brain, thymus and liver. In testis, expressed in both germ cells and Sertoli cells. Also expressed at low levels in the kidney. Expressed during neocortex and cerebellum development

Product Specifications

Recombinant Mouse NELL2-interacting cell ontogeny regulator 1 (Nicol1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 29-90aa, with C-terminal 6xHis-tagged tag, molecular weight 13.8kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

mRNA-binding protein which interacts with a range of target mRNAs including SERPINE1, ACTA2, CCN2 and COL4A1 and may promote extracellular matrix production (PubMed:38625739). Binds to the 3'-UTR of SERPINE1 mRNA and stabilizes the mRNA, possibly by competing for binding with SERBP1 and preventing SERBP1-mediated mRNA degradation (PubMed:38625739). Also binds to the 3'-UTR of ACTA2 (PubMed:38625739). Testis-derived lumicrine factor that triggers epididymal differentiation and sperm maturation (PubMed:37095084)

Subcellular Location

Secreted; Cytoplasm, perinuclear region

Tissue Specificity

Expression is enriched in both male and female reproductive organs, including the testis, epididymis, seminal vesicles, coagulating glands, ovary and uterus, and in various non-reproductive organs such as brain, thymus and liver (PubMed:37095084). In testis, expressed in both germ cells and Sertoli cells (PubMed:37095084). Also expressed at low levels in the kidney (PubMed:38625739). Expressed during neocortex and cerebellum development (PubMed:21287218)

Subunit

Interacts with NELL2; triggers epididymal differentiation (PubMed:37095084). Interacts with cell surface receptor TFRC; the interaction mediates uptake of NICOL1 into fibroblasts (PubMed:38625739)

Gene: Nicol1  |  Organism: Mus musculus  |  Synonyms: NELL2-interacting cofactor for lumicrine signaling

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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