Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial, Mycoplasma pneumoniae (strain ATCC 29342 / M129), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 32.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial

Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial — Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. Purity >85%.

SKU: BCRECP-003973 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-003973
Product DescriptionRecombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 487-718aa. [Accession | Q50341].
Uniprot No.Q50341
Gene Namesorf6
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region487-718aa
SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight32.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium and plays a major role in gliding motility. Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium. This protein and P1 mutually stabilize each other, and are required for formation of the attachment organelle.

Biological Context

Subcellular Location: Cell projection, attachment organelle membrane (Single-pass membrane protein); Cell surface

Product Specifications

Recombinant Mycoplasma pneumoniae Adhesin P40/P90 (orf6), partial is a recombinant protein from Mycoplasma pneumoniae (strain ATCC 29342 / M129), expressed in E.coli, covering amino acids 487-718aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 32.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Part of major adhesin Nap complex of the attachment organelle which mediates attachment to host ciliated epithelium (PubMed:1904124, PubMed:33057023) and plays a major role in gliding motility (PubMed:40153444). Binds sialylated oligosaccharides, which are present on the surface of its human host cells and which probably serve as the main host cell receptors for this bacterium (PubMed:33057023, PubMed:39226978). This protein and P1 mutually stabilize each other, and are required for formation of the attachment organelle (By similarity)

Subcellular Location

Cell projection, attachment organelle membrane (Single-pass membrane protein); Cell surface

Subunit

Forms a heterodimer with P1 (PubMed:33057023). After proteolysis P40 and P90 remain associated; under denaturing conditions P40 can be lost from the complex (PubMed:33057023). The Nap adhesion complex consists of 2 P1-P40/P90 heterodimers (By similarity)

Gene: orf6  |  Organism: Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top