Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated, Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 87.2kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated

Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated — Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. Purity >85%.

SKU: BCRECP-003989 Categories: , , ,
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Product Specifications

Product SkuBCRECP-003989
Product DescriptionRecombinant Hepatitis B virus genotype D subtype ayw Protein P (P) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 334-681aa. [Accession | Q67878].
Uniprot No.Q67878
Gene NamesP
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region334-681aa
SpeciesHepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight87.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid.

Product Specifications

Recombinant Hepatitis B virus genotype D subtype ayw Protein P (P), partial, Biotinylated is a recombinant protein from Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992) (HBV-D), expressed in E.coli, covering amino acids 334-681aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 87.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated together with the P protein, and reverse-transcribed inside the nucleocapsid. Initiation of reverse-transcription occurs first by binding the epsilon loop on the pgRNA genome, and is initiated by protein priming, thereby the 5'-end of (-)DNA is covalently linked to P protein. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA). The activity of P protein does not seem to be necessary for cccDNA generation, and is presumably released from (+)DNA by host nuclear DNA repair machinery

Gene: P  |  Organism: Hepatitis B virus genotype D subtype ayw (isolate Italy/CI/1992)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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