Recombinant Drosophila melanogaster Peptidylglycine alpha-hydroxylating monooxygenase (Phm), Drosophila melanogaster (Fruit fly), E.coli, C-terminal 6xHis-tagged, 45.1kDa, purity >90% as determined by SDS-PAGE.

Recombinant Drosophila melanogaster Peptidylglycine alpha-hydroxylating monooxygenase (Phm)

Recombinant Drosophila melanogaster Peptidylglycine alpha-hydroxylating monooxygenase (Phm) — Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Purity >90%.

SKU: BCRECP-004071 Category:
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Product Specifications

Product SkuBCRECP-004071
Product DescriptionRecombinant Drosophila melanogaster Peptidylglycine alpha-hydroxylating monooxygenase (Phm) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 25-365aa. [Accession | O01404].
Uniprot No.O01404
Gene NamesPhm
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region25-365aa
SpeciesDrosophila melanogaster (Fruit fly)
Tag InfoC-terminal 6xHis-tagged
Molecular weight45.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Produces an unstable peptidyl(2-hydroxyglycine) intermediate. C-terminal amidation of peptides is required for normal developmental transitions and for biosynthesis of secretory peptides throughout the life.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed in the central nervous system (CNS) in a small number of CNS neurons (approximately a few hundred). Expression is present both in cell bodies and within neuropil regions. It is strongly expressed in neuroendocrine neurons (at protein level)

Product Specifications

Recombinant Drosophila melanogaster Peptidylglycine alpha-hydroxylating monooxygenase (Phm) is a recombinant protein from Drosophila melanogaster (Fruit fly), expressed in E.coli, covering amino acids 25-365aa, with C-terminal 6xHis-tagged tag, molecular weight 45.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Monooxygenase that catalyzes an essential reaction in C-terminal alpha-amidation of peptides. Produces an unstable peptidyl(2-hydroxyglycine) intermediate. C-terminal amidation of peptides is required for normal developmental transitions and for biosynthesis of secretory peptides throughout the life

Subcellular Location

Secreted

Tissue Specificity

Expressed in the central nervous system (CNS) in a small number of CNS neurons (approximately a few hundred). Expression is present both in cell bodies and within neuropil regions. It is strongly expressed in neuroendocrine neurons (at protein level)

Gene: Phm  |  Organism: Drosophila melanogaster
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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