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Recombinant Mouse Serum paraoxonase/arylesterase 1 (Pon1)

Recombinant Mouse Serum paraoxonase/arylesterase 1 (Pon1) — Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Purity >85%.

SKU: BCRECP-004138 Categories: ,

Product Specifications

Product SkuBCRECP-004138
Product DescriptionRecombinant Mouse Serum paraoxonase/arylesterase 1 (Pon1) Protein is expressed from E.coli with Tag-Free. It contains 2-355aa. [Accession | P52430].
Uniprot No.P52430
Gene NamesPon1
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-355aa
SpeciesMus musculus (Mouse)
Tag InfoTag-Free
Molecular weight39.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasNeuroscience

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.

Biological Context

Subcellular Location: Secreted, extracellular space
Tissue Specificity: Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL

Product Specifications

Recombinant Mouse Serum paraoxonase/arylesterase 1 (Pon1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 2-355aa, with Tag-Free tag, molecular weight 39.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification

Subcellular Location

Secreted, extracellular space

Tissue Specificity

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL

Subunit

Homodimer. Interacts with CLU

Gene: Pon1  |  Organism: Mus musculus  |  Synonyms: Aromatic esterase 1; Serum aryldialkylphosphatase 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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