Recombinant Salmonella typhimurium Protein prgI (prgI), Biotinylated, Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 56.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Salmonella typhimurium Protein prgI (prgI), Biotinylated

Recombinant Salmonella typhimurium Protein prgI (prgI), Biotinylated — During infection, can induce innate immune responses. Purity >90%.

SKU: BCRECP-004161 Categories: ,
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Product Specifications

Product SkuBCRECP-004161
Product DescriptionRecombinant Salmonella typhimurium Protein prgI (prgI) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 1-80aa. [Accession | P41784].
Uniprot No.P41784
Gene NamesprgI
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-80aa
SpeciesSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight56.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

During infection, can induce innate immune responses. The needle proteins interact with host TLR2 or TLR4, and induce signaling by NF-kappa-B and/or AP-1. This activation is MyD88 dependent and results in increased expression of cytokines, including TNF, IL-6 and IL-8.

Biological Context

Subcellular Location: Secreted; Cell surface

Product Specifications

Recombinant Salmonella typhimurium Protein prgI (prgI), Biotinylated is a recombinant protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720), expressed in E.coli, covering amino acids 1-80aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 56.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

During infection, can induce innate immune responses (PubMed:24643544). The needle proteins interact with host TLR2 or TLR4, and induce signaling by NF-kappa-B and/or AP-1 (PubMed:24643544). This activation is MyD88 dependent and results in increased expression of cytokines, including TNF, IL-6 and IL-8 (PubMed:24643544)

Subcellular Location

Secreted; Cell surface

Subunit

The core secretion machinery of the T3SS is composed of approximately 20 different proteins, including cytoplasmic components, a base, an export apparatus and a needle (PubMed:30107569, PubMed:30242280, PubMed:31427728). This subunit polymerizes and forms the helical needle filament (PubMed:16501225, PubMed:20543831, PubMed:22699623, PubMed:30242280, PubMed:31260457, PubMed:31427728, PubMed:33750771, PubMed:34258394, PubMed:34706941). Interacts with the needle tip protein SipD/SctA (PubMed:21829362, PubMed:34706941). Interacts with the needle adapter protein PrgJ/SctI, the secretin InvG/SctC and the minor export apparatus protein SpaP/SctR (PubMed:33750771). In vitro, the needle protomer refolds spontaneously to extend the needle from the distal end (PubMed:20543831)

Gene: sctF1  |  Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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