Recombinant Streptococcus mitis Manganese ABC transporter substrate-binding lipoprotein (psaA), Streptococcus mitis, E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 40.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Streptococcus mitis Manganese ABC transporter substrate-binding lipoprotein (psaA)

Recombinant Streptococcus mitis Manganese ABC transporter substrate-binding lipoprotein (psaA) — Part of the ATP-riven (ABC) transport system PsaABC involved in manganese import. Purity >85%.

SKU: BCRECP-004184 Category:
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Product Specifications

Product SkuBCRECP-004184
Product DescriptionRecombinant Streptococcus mitis Manganese ABC transporter substrate-binding lipoprotein (psaA) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 20-309aa. [Accession | Q9L5X0].
Uniprot No.Q9L5X0
Gene NamespsaA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region20-309aa
SpeciesStreptococcus mitis
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight40.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Part of the ATP-riven (ABC) transport system PsaABC involved in manganese import. Binds manganese with high affinity and specificity and delivers it to the membrane permease for translocation into the cytoplasm. Also acts as an adhesin which is involved on adherence to extracellular matrix.

Biological Context

Subcellular Location: Cell membrane (Lipid-anchor)

Product Specifications

Recombinant Streptococcus mitis Manganese ABC transporter substrate-binding lipoprotein (psaA) is a recombinant protein from Streptococcus mitis, expressed in E.coli, covering amino acids 20-309aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 40.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Part of the ATP-riven (ABC) transport system PsaABC involved in manganese import (By similarity). Binds manganese with high affinity and specificity and delivers it to the membrane permease for translocation into the cytoplasm (By similarity). Also acts as an adhesin which is involved on adherence to extracellular matrix (By similarity)

Subcellular Location

Cell membrane (Lipid-anchor)

Gene: psaA  |  Organism: Streptococcus mitis  |  Synonyms: Pneumococcal surface adhesin A

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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