Recombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB), Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), E.coli, N-terminal 6xHis-tagged, 34.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB)

Recombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB) — One of the primary rRNA binding proteins. Purity >90%.

SKU: BCRECP-004284 Category:
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Product Specifications

Product SkuBCRECP-004284
Product DescriptionRecombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-280aa. [Accession | P9WHA5].
Uniprot No.P9WHA5
Gene NamesrplB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-280aa
SpeciesMycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Tag InfoN-terminal 6xHis-tagged
Molecular weight34.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation.

Product Specifications

Recombinant Mycobacterium tuberculosis 50S ribosomal protein L2 (rplB) is a recombinant protein from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv), expressed in E.coli, covering amino acids 1-280aa, with N-terminal 6xHis-tagged tag, molecular weight 34.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation

Subunit

Part of the 50S ribosomal subunit (PubMed:28977617, PubMed:35064151). Forms a bridge to the 30S subunit in the 70S ribosome

Gene: rplB  |  Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)  |  Synonyms: 50S ribosomal protein L2

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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