Recombinant Mouse NAD (+) hydrolase SARM1 (Sarm1), partial, Mus musculus (Mouse), E.coli, C-terminal 6xHis-tagged, 40.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse NAD (+) hydrolase SARM1 (Sarm1), partial

Recombinant Mouse NAD (+) hydrolase SARM1 (Sarm1), partial — NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Purity >90%.

SKU: BCRECP-004375 Categories: ,
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Product Specifications

Product SkuBCRECP-004375
Product DescriptionRecombinant Mouse NAD (+) hydrolase SARM1 (Sarm1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 409-705aa. [Accession | Q6PDS3].
Uniprot No.Q6PDS3
Gene NamesSarm1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region409-705aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight40.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction.

Biological Context

Subcellular Location: Cytoplasm; Cell projection, axon; Cell projection, dendrite; Synapse; Mitochondrion
Tissue Specificity: Widely expressed in the brain and neurons (at protein level). Expressed in photoreceptor cells of the neural retina

Product Specifications

Recombinant Mouse NAD (+) hydrolase SARM1 (Sarm1), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 409-705aa, with C-terminal 6xHis-tagged tag, molecular weight 40.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism (PubMed:25818290, PubMed:26686637, PubMed:27735788, PubMed:32312889). Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site (PubMed:21555464, PubMed:22678360, PubMed:25818290, PubMed:26423149, PubMed:26686637). Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction (PubMed:28334607). Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules (By similarity). Can activate neuronal cell death in response to stress (PubMed:19587044). Regulates dendritic arborization through the MAPK4-JNK pathway (PubMed:17724133, PubMed:21555464). Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38 (PubMed:21555464). Acts as a non-self dsDNA sensor in a sequence-independent manner to elicit NAD(+) degradation and thus to promote programmed cell death (By similarity)

Subcellular Location

Cytoplasm; Cell projection, axon; Cell projection, dendrite; Synapse; Mitochondrion

Tissue Specificity

Widely expressed in the brain and neurons (at protein level) (PubMed:21555464). Expressed in photoreceptor cells of the neural retina (PubMed:32312889)

Subunit

Homooctamer; forms an octameric ring via SAM domains (By similarity). Interacts with TICAM1/TRIF and thereby interferes with TICAM1/TRIF function (By similarity). Interacts with SDC2 (via cytoplasmic domain) and MAPK10/JNK3 (PubMed:17724133, PubMed:21555464)

Gene: Sarm1  |  Organism: Mus musculus  |  Synonyms: NADP(+) hydrolase SARM1; Sterile alpha and TIR motif-containing protein 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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