Recombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3), partial, Papio hamadryas (Hamadryas baboon), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 16.5kDa, purity >85% as determined by SDS-PAGE.

Recombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3), partial

Recombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3), partial — Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Purity >85%.

SKU: BCRECP-004458 Category:
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Product Specifications

Product SkuBCRECP-004458
Product DescriptionRecombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 87-166aa. [Accession | B6VH79].
Uniprot No.B6VH79
Gene NamesSPACA3
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region87-166aa
SpeciesPapio hamadryas (Hamadryas baboon)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight16.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasDevelopmental Biology

Function

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro.

Biological Context

Subcellular Location: Cytoplasmic vesicle, secretory vesicle, acrosome membrane (Single-pass type II membrane protein)

Product Specifications

Recombinant Papio hamadryas Sperm acrosome membrane-associated protein 3 (SPACA3), partial is a recombinant protein from Papio hamadryas (Hamadryas baboon), expressed in E.coli, covering amino acids 87-166aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 16.5kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. It could be a potential receptor for the egg oligosaccharide residue N-acetylglucosamine, which is present in the extracellular matrix over the egg plasma membrane. The processed form has no detectable bacteriolytic activity in vitro (By similarity)

Subcellular Location

Cytoplasmic vesicle, secretory vesicle, acrosome membrane (Single-pass type II membrane protein)

Subunit

Interacts with ASTL

Gene: SPACA3  |  Organism: Papio hamadryas  |  Synonyms: Sperm protein reactive with antisperm antibodies

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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