Recombinant Staphylococcus aureus Staphopain B (sspB), Staphylococcus aureus (strain Mu50 / ATCC 700699), Yeast, N-terminal 6xHis-tagged, 21.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Staphopain B (sspB)

Recombinant Staphylococcus aureus Staphopain B (sspB) — Cysteine protease that plays an important role in the inhibition of host innate immune response. Purity >90%.

SKU: BCRECP-004488 Category:
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Product Specifications

Product SkuBCRECP-004488
Product DescriptionRecombinant Staphylococcus aureus Staphopain B (sspB) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 220-393aa. [Accession | Q99V46].
Uniprot No.Q99V46
Gene NamessspB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region220-393aa
SpeciesStaphylococcus aureus (strain Mu50 / ATCC 700699)
Tag InfoN-terminal 6xHis-tagged
Molecular weight21.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Degrades host elastin, fibrogen, fibronectin and kininogen. Blocks phagocytosis of opsonised S.aureus by neutrophils and monocytes by inducing their death in a proteolytic activity-dependent manner.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Staphylococcus aureus Staphopain B (sspB) is a recombinant protein from Staphylococcus aureus (strain Mu50 / ATCC 700699), expressed in Yeast, covering amino acids 220-393aa, with N-terminal 6xHis-tagged tag, molecular weight 21.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Cysteine protease that plays an important role in the inhibition of host innate immune response. Degrades host elastin, fibrogen, fibronectin and kininogen. Blocks phagocytosis of opsonised S.aureus by neutrophils and monocytes by inducing their death in a proteolytic activity-dependent manner. Decreases surface expression of the 'don't eat me' signal CD31 on neutrophils. Cleaves host galectin-3/LGALS3, thereby inhibiting the neutrophil-activating ability of the lectin

Subcellular Location

Secreted

Subunit

In the cytoplasm, prematurely activated/folded SspB forms a stable non-covalent complex with SspC

Gene: sspB  |  Organism: Staphylococcus aureus (strain Mu50 / ATCC 700699)  |  Synonyms: Staphylococcal cysteine proteinase B; Staphylopain B

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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