Recombinant Rat Protransforming growth factor alpha (Tgfa), Rattus norvegicus (Rat), E.coli, N-terminal 6xHis-KSI-tagged, 30.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Rat Protransforming growth factor alpha (Tgfa)

Recombinant Rat Protransforming growth factor alpha (Tgfa) — recombinant protein from Rattus norvegicus (Rat), expressed in E.coli. Purity >90%.

SKU: BCRECP-004551 Categories: ,
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Product Specifications

Product SkuBCRECP-004551
Product DescriptionRecombinant Rat Protransforming growth factor alpha (Tgfa) Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 24-159aa. [Accession | P01134].
Uniprot No.P01134
Gene NamesTgfa
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region24-159aa
SpeciesRattus norvegicus (Rat)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight30.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.

Biological Context

Subcellular Location: Cell membrane (Single-pass type I membrane protein)

Product Specifications

Recombinant Rat Protransforming growth factor alpha (Tgfa) is a recombinant protein from Rattus norvegicus (Rat), expressed in E.coli, covering amino acids 24-159aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 30.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar

Subcellular Location

Cell membrane (Single-pass type I membrane protein)

Subunit

Interacts with the PDZ domains of MAGI3, SDCBP and SNTA1. The interaction with SDCBP, is required for the targeting to the cell surface. In the endoplasmic reticulum, in its immature form (i.e. with a prosegment and lacking full N-glycosylation), interacts with CNIH. In the Golgi apparatus, may form a complex with CNIH and GORASP2. Interacts (via cytoplasmic C-terminal domain) with NKD2 (By similarity)

Gene: Tgfa  |  Organism: Rattus norvegicus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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