Recombinant Clostridioides difficile Toxin A (toxA), partial, Clostridioides difficile (Peptoclostridium difficile), E.coli, N-terminal 6xHis-tagged, 40.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Clostridioides difficile Toxin A (toxA), partial

Recombinant Clostridioides difficile Toxin A (toxA), partial — Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases. Purity >85%.

SKU: BCRECP-004594 Category:
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Product Specifications

Product SkuBCRECP-004594
Product DescriptionRecombinant Clostridioides difficile Toxin A (toxA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2387-2710aa. [Accession | P16154].
Uniprot No.P16154
Gene NamestoxA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2387-2710aa
SpeciesClostridioides difficile (Peptoclostridium difficile)
Tag InfoN-terminal 6xHis-tagged
Molecular weight40.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases. Acts by mediating monoglucosylation of small GTPases of the Rho family (Rac1, RhoA, RhoB, RhoC, Rap2A and Cdc42) in host cells at the conserved threonine residue located in the switch I region ('Thr-37/35'), using UDP-alpha-D-glucose as the sugar donor. Monoglucosylation of host small GTPases completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function.

Biological Context

Subcellular Location: Host cytoplasm, host cytosol; Host cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Clostridioides difficile Toxin A (toxA), partial is a recombinant protein from Clostridioides difficile (Peptoclostridium difficile), expressed in E.coli, covering amino acids 2387-2710aa, with N-terminal 6xHis-tagged tag, molecular weight 40.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases (PubMed:22267739, PubMed:22747490, PubMed:24905543, PubMed:30622517, PubMed:7775453). Acts by mediating monoglucosylation of small GTPases of the Rho family (Rac1, RhoA, RhoB, RhoC, Rap2A and Cdc42) in host cells at the conserved threonine residue located in the switch I region ('Thr-37/35'), using UDP-alpha-D-glucose as the sugar donor (PubMed:22267739, PubMed:22747490, PubMed:24905543, PubMed:30622517, PubMed:7775453). Monoglucosylation of host small GTPases completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function (PubMed:7775453). Also able to catalyze monoglucosylation of some members of the Ras family (H-Ras/HRAS, K-Ras/KRAS and N-Ras/NRAS), but with much less efficiency than with Rho proteins, suggesting that it does not act on Ras proteins in vivo (PubMed:30622517)

Subcellular Location

Host cytoplasm, host cytosol; Host cell membrane (Peripheral membrane protein)

Subunit

Interacts with host LDLR; LDLR probably does not constitute a major receptor but may contribute to entry into cells

Gene: tcdA  |  Organism: Clostridioides difficile
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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