Recombinant Ixodes scapularis Tick receptor for ospA (TROSPA), Ixodes scapularis (Black-legged tick) (Deer tick), E.coli, N-terminal 6xHis-tagged, 20.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Ixodes scapularis Tick receptor for ospA (TROSPA)

Recombinant Ixodes scapularis Tick receptor for ospA (TROSPA) — Serves as a receptor for ospA protein of B. Purity >90%.

SKU: BCRECP-004625 Category:
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Product Specifications

Product SkuBCRECP-004625
Product DescriptionRecombinant Ixodes scapularis Tick receptor for ospA (TROSPA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-161aa. [Accession | Q5SDL7].
Uniprot No.Q5SDL7
Gene NamesTROSPA
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-161aa
SpeciesIxodes scapularis (Black-legged tick) (Deer tick)
Tag InfoN-terminal 6xHis-tagged
Molecular weight20.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Serves as a receptor for ospA protein of B.burgdorferi, the Lyme disease agent. Required for spirochetal colonization. Essential for pathogen adherence to the vector.

Biological Context

Subcellular Location: Cell membrane
Tissue Specificity: Specifically expressed in gut. Localizes predominantly in the intercellular spaces and luminal surface of the gut. In the gut, it localizes along tight junctions. Not expressed in salivary gland or hemolymph

Product Specifications

Recombinant Ixodes scapularis Tick receptor for ospA (TROSPA) is a recombinant protein from Ixodes scapularis (Black-legged tick) (Deer tick), expressed in E.coli, covering amino acids 1-161aa, with N-terminal 6xHis-tagged tag, molecular weight 20.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Serves as a receptor for ospA protein of B.burgdorferi, the Lyme disease agent. Required for spirochetal colonization. Essential for pathogen adherence to the vector

Subcellular Location

Cell membrane

Tissue Specificity

Specifically expressed in gut. Localizes predominantly in the intercellular spaces and luminal surface of the gut. In the gut, it localizes along tight junctions. Not expressed in salivary gland or hemolymph

Subunit

Interacts with ospA protein from B.burgdorferi

Gene: TROSPA  |  Organism: Ixodes scapularis

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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