Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE), Escherichia coli (strain K12), E.coli, Tag-Free, 16.9kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE)

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE) — Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Purity >90%.

SKU: BCRECP-004634 Category:
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Product Specifications

Product SkuBCRECP-004634
Product DescriptionRecombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE) Protein is expressed from E.coli with Tag-Free. It contains 1-153aa. [Accession | P0AF67].
Uniprot No.P0AF67
Gene NamestsaE
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-153aa
SpeciesEscherichia coli (strain K12)
Tag InfoTag-Free
Molecular weight16.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasStreptomyces viridochromogenes

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD.

Biological Context

Subcellular Location: Cytoplasm

Product Specifications

Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE) is a recombinant protein from Escherichia coli (strain K12), expressed in E.coli, covering amino acids 1-153aa, with Tag-Free tag, molecular weight 16.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro

Subcellular Location

Cytoplasm

Subunit

Homodimer. Interacts with TsaB

Gene: tsaE  |  Organism: Escherichia coli (strain K12)  |  Synonyms: t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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