Recombinant Hepatitis B virus genotype D Protein X (X), Hepatitis B virus genotype D (isolate Germany/1-91/1991) (HBV-D), E.coli, N-terminal 6xHis-SUMO-tagged, 32.7kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Hepatitis B virus genotype D Protein X (X)

Recombinant Hepatitis B virus genotype D Protein X (X) — Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Purity >90%.

SKU: BCRECP-004753 Categories: , ,
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Product Specifications

Product SkuBCRECP-004753
Product DescriptionRecombinant Hepatitis B virus genotype D Protein X (X) Protein is expressed from E.coli with N-terminal 6xHis-SUMO-tagged. It contains 1-154aa. [Accession | O93195].
Uniprot No.O93195
Gene NamesX
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-154aa
SpeciesHepatitis B virus genotype D (isolate Germany/1-91/1991) (HBV-D)
Tag InfoN-terminal 6xHis-SUMO-tagged
Molecular weight32.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma).

Biological Context

Subcellular Location: Host cytoplasm; Host nucleus; Host mitochondrion

Product Specifications

Recombinant Hepatitis B virus genotype D Protein X (X) is a recombinant protein from Hepatitis B virus genotype D (isolate Germany/1-91/1991) (HBV-D), expressed in E.coli, covering amino acids 1-154aa, with N-terminal 6xHis-SUMO-tagged tag, molecular weight 32.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Multifunctional protein that plays a role in silencing host antiviral defenses and promoting viral transcription. Does not seem to be essential for HBV infection. May be directly involved in development of cirrhosis and liver cancer (hepatocellular carcinoma). Most of cytosolic activities involve modulation of cytosolic calcium. The effect on apoptosis is controversial depending on the cell types in which the studies have been conducted. May induce apoptosis by localizing in mitochondria and causing loss of mitochondrial membrane potential. May also modulate apoptosis by binding host CFLAR, a key regulator of the death-inducing signaling complex (DISC). Promotes viral transcription by using the host E3 ubiquitin ligase DDB1 to target the SMC5-SMC6 complex to proteasomal degradation. This host complex would otherwise bind to viral episomal DNA, and prevents its transcription. Moderately stimulates transcription of many different viral and cellular transcription elements. Promoters and enhancers stimulated by HBx contain DNA binding sites for NF-kappa-B, AP-1, AP-2, c-EBP, ATF/CREB, or the calcium-activated factor NF-AT

Subcellular Location

Host cytoplasm; Host nucleus; Host mitochondrion

Subunit

May form homodimer. May interact with host CEBPA, CFLAR, CREB1, DDB1, E4F1, HBXIP, HSPD1/HSP60, NFKBIA, POLR2E and SMAD4. Interacts with host SMC5-SMC6 complex and induces its degradation. Interacts with host TRPC4AP; leading to prevent ubiquitination of TRPC4AP. Interacts with host PLSCR1; this interaction promotes ubiquitination and degradation of HBx and impairs HBx-mediated cell proliferation

Gene: X  |  Organism: Hepatitis B virus genotype D (isolate Germany/1-91/1991)  |  Synonyms: HBx; Peptide X; pX

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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