Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5), Escherichia phage T7 (Bacteriophage T7), Yeast, Tag-Free, 27.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5)

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) — Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks. Purity >90%.

SKU: BCRECP-001957 Category:
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Product Specifications

Product SkuBCRECP-001957
Product DescriptionRecombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) Protein is expressed from Yeast with Tag-Free. It contains 1-232aa. [Accession | P03696].
Uniprot No.P03696
Gene Names2.5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-232aa
SpeciesEscherichia phage T7 (Bacteriophage T7)
Tag InfoTag-Free
Molecular weight27.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks. Coats the lagging-strand ssDNA as the replication fork advances and stimulates the activities of viral DNA polymerase and primase/helicase. Coordinates simultaneous synthesis of leading- and lagging-strands.

Product Specifications

Recombinant Escherichia phage T7 Single-stranded DNA-binding protein (2.5) is a recombinant protein from Escherichia phage T7 (Bacteriophage T7), expressed in Yeast, covering amino acids 1-232aa, with Tag-Free tag, molecular weight 27.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, formation of concatemers, recombination and repair of double-stranded breaks (PubMed:11222583, PubMed:16807232, PubMed:8617248, PubMed:9079662). Coats the lagging-strand ssDNA as the replication fork advances and stimulates the activities of viral DNA polymerase and primase/helicase (PubMed:8617248). Coordinates simultaneous synthesis of leading- and lagging-strands (PubMed:9651583). Together with DNA primase/helicase, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange (PubMed:8617248). Also promotes the formation of joint molecules (PubMed:8617248, PubMed:9079662). Disrupts loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation (PubMed:1634538)

Subunit

Homodimer (PubMed:11481454, PubMed:1634538). Interacts (via C-terminus) with the viral DNA polymerase (PubMed:12766155, PubMed:15795374, PubMed:1634539, PubMed:8106511). Interacts with the viral helicase/primase (PubMed:1634539). Part of the replicase complex that includes the DNA polymerase, host thioredoxin, the primase/helicase and the single-stranded DNA binding protein (PubMed:22977246)

Organism: Escherichia phage T7  |  Synonyms: 2.5 protein; Gene product 2.5
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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