Recombinant Escherichia phage RB69 Gp32 (32), Escherichia phage RB69 (Bacteriophage RB69), E.coli, N-terminal 6xHis-tagged, 37.2kDa, purity >90% as determined by SDS-PAGE.

Recombinant Escherichia phage RB69 Gp32 (32)

Recombinant Escherichia phage RB69 Gp32 (32) — Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Purity >90%.

SKU: BCRECP-001962 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-001962
Product DescriptionRecombinant Escherichia phage RB69 Gp32 (32) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 1-299aa. [Accession | Q7Y265].
Uniprot No.Q7Y265
Gene Names32
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-299aa
SpeciesEscherichia phage RB69 (Bacteriophage RB69)
Tag InfoN-terminal 6xHis-tagged
Molecular weight37.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and the replicative helicase, probably via its interaction with the helicase assembly factor.

Product Specifications

Recombinant Escherichia phage RB69 Gp32 (32) is a recombinant protein from Escherichia phage RB69 (Bacteriophage RB69), expressed in E.coli, covering amino acids 1-299aa, with N-terminal 6xHis-tagged tag, molecular weight 37.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Single-stranded DNA-binding protein that participates in viral DNA replication, recombination, and repair. Coats the lagging-strand ssDNA as the replication fork advances. Stimulates the activities of viral DNA polymerase and the replicative helicase, probably via its interaction with the helicase assembly factor. Together with the replicative helicase and the helicase assembly factor, promotes pairing of two homologous DNA molecules containing complementary single-stranded regions and mediates homologous DNA strand exchange. Promotes also the formation of joint molecules. mRNA specific autogenous translational repressor

Subunit

Homodimer in the absence of DNA, monomer when binding DNA. Interacts with the DNA helicase assembly protein; a ternary complex between the helicase assembly protein, the single-stranded DNA-binding protein and ssDNA is an obligatory intermediate in the helicase loading mechanism. Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, the primase, the replicative helicase and the helicase assembly factor. Interacts (via C-terminus) with the viral SF1 dDA helicase. Interacts with the viral SF2 UvsW repair helicase

Gene: 32  |  Organism: Escherichia phage RB69  |  Synonyms: Helix-destabilizing protein

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top