Recombinant Mouse Allograft inflammatory factor 1 (Aif1), Mus musculus (Mouse), Mammalian cell, C-terminal hFc1-tagged, 45.7kDa, purity >90% as determined by SDS-PAGE.

Recombinant Mouse Allograft inflammatory factor 1 (Aif1)

Recombinant Mouse Allograft inflammatory factor 1 (Aif1) — Actin-binding protein that enhances membrane ruffling and RAC activation. Purity >90%.

SKU: BCRECP-002042 Category:
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Product Specifications

Product SkuBCRECP-002042
Product DescriptionRecombinant Mouse Allograft inflammatory factor 1 (Aif1) Protein is expressed from Mammalian cell with C-terminal hFc1-tagged. It contains 2-147aa. [Accession | O70200].
Uniprot No.O70200
Gene NamesAif1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region2-147aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal hFc1-tagged
Molecular weight45.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium.

Biological Context

Subcellular Location: Cytoplasm, cytoskeleton; Cell projection, ruffle membrane (Peripheral membrane protein); Cell projection, phagocytic cup
Tissue Specificity: Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages

Product Specifications

Recombinant Mouse Allograft inflammatory factor 1 (Aif1) is a recombinant protein from Mus musculus (Mouse), expressed in Mammalian cell, covering amino acids 2-147aa, with C-terminal hFc1-tagged tag, molecular weight 45.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation

Subcellular Location

Cytoplasm, cytoskeleton; Cell projection, ruffle membrane (Peripheral membrane protein); Cell projection, phagocytic cup

Tissue Specificity

Abundantly expressed in the testis, moderately in the spleen and lymph nodes and at low levels in the liver and thymus. Detected in macrophages

Subunit

Homodimer (Potential). Monomer. Interacts with LCP1

Gene: Aif1  |  Organism: Mus musculus  |  Synonyms: Ionized calcium-binding adapter molecule 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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