Recombinant Dog Brain-derived neurotrophic factor (BDNF), Canis lupus familiaris (Dog) (Canis familiaris), E.coli, N-terminal 6xHis-tagged, 19.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Dog Brain-derived neurotrophic factor (BDNF)

Recombinant Dog Brain-derived neurotrophic factor (BDNF) — Important signaling molecule that activates signaling cascades downstream of NTRK2. Purity >90%.

SKU: BCRECP-002189 Categories: ,
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Product Specifications

Product SkuBCRECP-002189
Product DescriptionRecombinant Dog Brain-derived neurotrophic factor (BDNF) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 129-247aa. [Accession | Q7YRB4].
Uniprot No.Q7YRB4
Gene NamesBDNF
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region129-247aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoN-terminal 6xHis-tagged
Molecular weight19.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCardiovascular

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Dog Brain-derived neurotrophic factor (BDNF) is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in E.coli, covering amino acids 129-247aa, with N-terminal 6xHis-tagged tag, molecular weight 19.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity)

Subcellular Location

Secreted

Subunit

Monomers and homodimers (By similarity). Binds to NTRK2/TRKB. Can form heterodimers with other neurotrophin family members, such as NTF3 and NTF4 (in vitro), but the physiological relevance of this is not clear (By similarity). BDNF precursor form: interacts with the heterodimer formed by NGFR and SORCS2. Mature BDNF has much lower affinity for the heterodimer formed by NGFR and SORCS2 (By similarity)

Gene: BDNF  |  Organism: Canis lupus familiaris  |  Synonyms: Brain-derived neurotrophic factor

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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