Recombinant Mouse CD81 antigen (Cd81), partial, Mus musculus (Mouse), E.coli, N-terminal 6xHis-GST-tagged, 40.9kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Mouse CD81 antigen (Cd81), partial

Recombinant Mouse CD81 antigen (Cd81), partial — recombinant protein from Mus musculus (Mouse), expressed in E.coli. Purity >90%.

SKU: BCRECP-002331 Categories: ,
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Product Specifications

Product SkuBCRECP-002331
Product DescriptionRecombinant Mouse CD81 antigen (Cd81) Protein is expressed from E.coli with N-terminal 6xHis-GST-tagged. It contains 116-201aa. [Accession | P35762].
Uniprot No.P35762
Gene NamesCd81
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region116-201aa
SpeciesMus musculus (Mouse)
Tag InfoN-terminal 6xHis-GST-tagged
Molecular weight40.9kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasImmunology

Function

(Microbial infection) Specifically required for Plasmodium yoelii infectivity of hepatocytes, controlling sporozoite entry in hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms.

Biological Context

Subcellular Location: Cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in oocytes (at protein level). Highly expressed in granulosa cells. Expressed in skeletal muscle mainly in endothelial cells of endomysial capillaries, in satellite cells and myoblasts (at protein level). Expressed in hepatocytes (at protein level)

Product Specifications

Recombinant Mouse CD81 antigen (Cd81), partial is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 116-201aa, with N-terminal 6xHis-GST-tagged tag, molecular weight 40.9kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

(Microbial infection) Specifically required for Plasmodium yoelii infectivity of hepatocytes, controlling sporozoite entry in hepatocytes via the parasitophorous vacuole and subsequent parasite differentiation to exoerythrocytic forms

Subcellular Location

Cell membrane (Multi-pass membrane protein); Basolateral cell membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in oocytes (at protein level) (PubMed:16380109, PubMed:17290409, PubMed:23213457). Highly expressed in granulosa cells (PubMed:16380109). Expressed in skeletal muscle mainly in endothelial cells of endomysial capillaries, in satellite cells and myoblasts (at protein level) (PubMed:23575678). Expressed in hepatocytes (at protein level) (PubMed:12483205)

Subunit

Homodimer (By similarity). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B cells. Interacts (via the second extracellular domain) with CD19; this interaction is initiated early during biosynthesis in the ER and enables trafficking of only properly folded CD19 (By similarity). Part of a complex that includes MHC class II/HLA-DR molecules and IFITM1. Interacts with IFITM1 (By similarity). Interacts with IFITM2 and IFITM3 (PubMed:16395393). Part of integrin-tetraspanin complex composed of CD9, CD81, beta-1 and beta-2 integrins in the membrane of monocyte/macrophages (By similarity). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (By similarity). Interacts with CD247/CD3 zeta, ICAM1 and CD9 at the immune synapse on T cell membrane (By similarity). Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, possibly CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (By similarity). Part of a complex composed of CD9, CD81, PTGFRN and IGSF8 (PubMed:23575678). Interacts directly with IGSF8 (PubMed:11673522). Interacts with CD53 and SCIMP (By similarity). Interacts with SAMHD1 (via its C-terminus) (By similarity). Interacts with glypican GPC3 and with the transcriptional repressor HHEX; binding to GPC3 decreases the availability of free CD81 for binding to HHEX, resulting in nuclear translocation of HHEX and transcriptional repression (PubMed:23665349). Interacts with CLDN1 (By similarity). Interacts with CLDN6 and CLDN9 (By similarity)

Gene: Cd81  |  Organism: Mus musculus  |  Synonyms: 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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