Recombinant Saccharomyces cerevisiae Cell wall mannoprotein CIS3 (CIS3), Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 24.3kDa, purity >90% as determined by SDS-PAGE.

Recombinant Saccharomyces cerevisiae Cell wall mannoprotein CIS3 (CIS3)

Recombinant Saccharomyces cerevisiae Cell wall mannoprotein CIS3 (CIS3) — Component of the outer cell wall layer. Purity >90%.

SKU: BCRECP-002380 Category:
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Product Specifications

Product SkuBCRECP-002380
Product DescriptionRecombinant Saccharomyces cerevisiae Cell wall mannoprotein CIS3 (CIS3) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 65-227aa. [Accession | P47001].
Uniprot No.P47001
Gene NamesCIS3
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region65-227aa
SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight24.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents.

Biological Context

Subcellular Location: Secreted, cell wall

Product Specifications

Recombinant Saccharomyces cerevisiae Cell wall mannoprotein CIS3 (CIS3) is a recombinant protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast), expressed in E.coli, covering amino acids 65-227aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 24.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Component of the outer cell wall layer. Required for stability of the cell wall and for optimal growth. Required for resistance against several antifungal and cell wall-perturbing agents

Subcellular Location

Secreted, cell wall

Gene: CIS3  |  Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c)  |  Synonyms: Covalently-linked cell wall protein 5/11; Protein with internal repeats 4; Soluble cell wall protein 8
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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