Recombinant Staphylococcus aureus Clumping factor B(clfB) , partial, Staphylococcus aureus (strain Newman), Yeast, N-terminal 6xHis-tagged, 56.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Staphylococcus aureus Clumping factor B(clfB) , partial

Recombinant Staphylococcus aureus Clumping factor B(clfB) , partial — Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Purity >85%.

SKU: BCRECP-002391 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-002391
Product DescriptionRecombinant Staphylococcus aureus Clumping factor B(clfB) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 45-542aa. [Accession | O86476].
Uniprot No.O86476
Gene NamesclfB
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region45-542aa
SpeciesStaphylococcus aureus (strain Newman)
Tag InfoN-terminal 6xHis-tagged
Molecular weight56.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Partly responsible for mediating bacterial attachment to the highly keratinized squamous epithelial cells from the nasal cavity via an interaction with cytokeratin K10 (K10). Also promotes bacterial attachment to cultured keratinocytes, possibly through an interaction with cytokeratin K10.

Biological Context

Subcellular Location: Secreted, cell wall (Peptidoglycan-anchor)

Product Specifications

Recombinant Staphylococcus aureus Clumping factor B(clfB) , partial is a recombinant protein from Staphylococcus aureus (strain Newman), expressed in Yeast, covering amino acids 45-542aa, with N-terminal 6xHis-tagged tag, molecular weight 56.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Partly responsible for mediating bacterial attachment to the highly keratinized squamous epithelial cells from the nasal cavity via an interaction with cytokeratin K10 (K10). Also promotes bacterial attachment to cultured keratinocytes, possibly through an interaction with cytokeratin K10. Binds mouse cytokeratin K10. Activates human platelet aggregation

Subcellular Location

Secreted, cell wall (Peptidoglycan-anchor)

Gene: clfB  |  Organism: Staphylococcus aureus (strain Newman)  |  Synonyms: Fibrinogen receptor B; Fibrinogen-binding protein B
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top