Recombinant Mouse Cold shock domain-containing protein E1 (Csde1)

Recombinant Mouse Cold shock domain-containing protein E1 (Csde1) — RNA-binding protein involved in translationally coupled mRNA turnover. Purity >90%.

SKU: BCRECP-002448 Category:

Product Specifications

Product SkuBCRECP-002448
Product DescriptionRecombinant Mouse Cold shock domain-containing protein E1 (Csde1) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 1-798aa. [Accession | Q91W50].
Uniprot No.Q91W50
Gene NamesCsde1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-798aa
SpeciesMus musculus (Mouse)
Tag InfoC-terminal 6xHis-tagged
Molecular weight95.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasEpigenetics and Nuclear Signaling

Function

RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules.

Biological Context

Subcellular Location: Cytoplasm; Cytoplasm, Stress granule; Cytoplasm, P-body

Product Specifications

Recombinant Mouse Cold shock domain-containing protein E1 (Csde1) is a recombinant protein from Mus musculus (Mouse), expressed in E.coli, covering amino acids 1-798aa, with C-terminal 6xHis-tagged tag, molecular weight 95.7kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Protein Biology

Function

RNA-binding protein involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules

Subcellular Location

Cytoplasm; Cytoplasm, Stress granule; Cytoplasm, P-body

Subunit

Component of a multi subunit autoregulatory ribonucleoprotein complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1. Interacts with STRAP. Part of a complex associated with the FOS mCRD domain and consisting of PABPC1, PAIP1, HNRPD and SYNCRIP. The interaction with PABPC1 is direct and RNA-independent. Interacts with EIF4ENIF1/4E-T

Gene: Csde1  |  Organism: Mus musculus

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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