Mouse anti-Human/Mouse/Rat sapiens (Human) PPP4C Monoclonal Antibody

Function

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin

Subcellular Location

Cytoplasm; Nucleus; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Subunit

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 (PubMed:12668731, PubMed:16085932, PubMed:18614045, PubMed:18715871). The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 (PubMed:12668731). Interacts with REL, NFKB1/p50 and RELA (PubMed:1336397). Interacts with SMN1 and GEMIN4 (PubMed:12668731). Interacts with IRS4 (phosphorylated) (PubMed:15331607). Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 (PubMed:16085932). Interacts with HDAC3 (PubMed:15805470)

• Brewis N.D. et al. Protein phosphatase X has been highly conserved during mammalian evolution. — Biochim. Biophys. Acta (1992)
• Cohen P.T.W. et al. PubMed: CI-1UTGM5MG6KQCC (DEC-)
• Hu M.C.-T. et al. Protein phosphatase X interacts with c-Rel and stimulates c-Rel/nuclear factor kappaB activity. — J. Biol. Chem. (1998)
• The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). — Genome Res. (2004)
• Zhou G. et al. Protein phosphatase 4 is involved in tumor necrosis factor-alpha-induced activation of c-Jun N-terminal kinase. — J. Biol. Chem. (2002)
• Mourtada-Maarabouni M. et al. Functional expression cloning reveals proapoptotic role for protein phosphatase 4. — Cell Death Differ. (2003)
• Carnegie G.K. et al. Protein phosphatase 4 interacts with the survival of motor neurons complex and enhances the temporal localisation of snRNPs. — J. Cell Sci. (2003)
• Mihindukulasuriya K.A. et al. Protein phosphatase 4 interacts with and down-regulates insulin receptor substrate 4 following tumor necrosis factor-alpha stimulation. — J. Biol. Chem. (2004)