Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase (AER), Arabidopsis thaliana (Mouse-ear cress), E.coli, Tag-Free, 38.1kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase (AER)

Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase (AER) — Involved in the detoxification of reactive carbonyls. Purity >90%.

SKU: BCRECP-002026 Categories: ,
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Product Specifications

Product SkuBCRECP-002026
Product DescriptionRecombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase (AER) Protein is expressed from E.coli with Tag-Free. It contains 1-345aa. [Accession | Q39172].
Uniprot No.Q39172
Gene NamesAER
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-345aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoTag-Free
Molecular weight38.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Involved in the detoxification of reactive carbonyls. Acts on lipid peroxide-derived reactive aldehydes. Specific to a double bond activated by an adjacent carbonyl group.

Biological Context

Subcellular Location: Cytoplasm; Nucleus, nucleoplasm
Tissue Specificity: Expressed in leaves

Product Specifications

Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase (AER) is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in E.coli, covering amino acids 1-345aa, with Tag-Free tag, molecular weight 38.1kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Involved in the detoxification of reactive carbonyls (PubMed:10848984, PubMed:12514241, PubMed:16299173). Acts on lipid peroxide-derived reactive aldehydes (PubMed:12514241). Specific to a double bond activated by an adjacent carbonyl group (PubMed:12514241). Can use both quinones and diamide as substrates, but not menadione, ferricyanide or phylloquinone (PubMed:10848984). Can use 4-hydroxy-(2E)-nonenal (HNE), 4-hydroxy-(2E)-hexenal (HHE), (2E)-nonenal, (2E)-hexenal, (2E)-pentenal, propenal (acrolein), 3-buten-2-one and 3-penten-2-one, but not (R)-(-)-carvone, n-nonanal, n-hexanal, (3Z)-hexanal, cyclohex-2-en-1-one or 12-oxo phytodienoic acid (OPDA) as electron acceptors (PubMed:12514241). Catalyzes the reduction of the alpha,beta-unsaturated bond of 2-alkenals, of lipid peroxide-derived oxenes 9-oxo-10(E),12(Z)-octadecadienoic acid (9-KODE) and 13-oxo-9(Z),11(E)-octadecadienoic acid (13-KODE), as well as 4-oxo-(2E)-nonenal and 4-hydroxynonenal (PubMed:16299173). Can use 12-oxo-10(E) dodecanoate (traumatin), trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy-(10E)-octadecenoic acid (trans-EKODE-1b) and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates (PubMed:26678323). Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro) (PubMed:17028190). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro) (PubMed:17028190). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis (PubMed:17028190)

Subcellular Location

Cytoplasm; Nucleus, nucleoplasm

Tissue Specificity

Expressed in leaves

Subunit

Homodimer

Gene: AER  |  Organism: Arabidopsis thaliana  |  Synonyms: NADP-dependent alkenal double bond reductase P1; NADPH-azodicarbonyl/quinone reductase; NADPH:2-alkenal/one alpha,beta-hydrogenase; P1-zeta-crystallin protein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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