Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein — Required for male fertility. Purity >95%.

SKU: BCRECP-00550TM Category:

Product Specifications

Uniprot No.F4JP36
Gene NamesHAP2
PurityGreater than 95% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region25-705aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoC-terminal 10xHis-tagged
Molecular weight81.0kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass membrane protein); Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed only in mature pollen, in the two sperm cells

Product Specifications

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in in vitro E.coli expression system, covering amino acids 25-705aa, with C-terminal 10xHis-tagged tag, molecular weight 81.0kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not Test

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

Protein Biology

Function

Required for male fertility (PubMed:17079265, PubMed:20333238). Plays a role in pollen tube guidance and successful gamete attachment (PubMed:17079265). Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm (PubMed:17079265, PubMed:20333238, PubMed:21209845). Mediates the fusion of cell membranes (PubMed:28137780). Not required for pollen tube outgrowth (PubMed:17079265, PubMed:20333238)

Subcellular Location

Endoplasmic reticulum membrane (Single-pass membrane protein); Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Expressed only in mature pollen, in the two sperm cells

Gene: HAP2  |  Organism: Arabidopsis thaliana  |  Synonyms: GENERATIVE CELL SPECIFIC 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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