Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein, Arabidopsis thaliana (Mouse-ear cress), in vitro E.coli expression system, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 80.7kDa, purity >85% as determined by SDS-PAGE.

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein — Required for male fertility. Purity >85%.

SKU: BCRECP-00374TM Category:
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Product Specifications

Uniprot No.F4JP36
Gene NamesHAP2
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region25-705aa
SpeciesArabidopsis thaliana (Mouse-ear cress)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight80.7kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Required for male fertility. Plays a role in pollen tube guidance and successful gamete attachment. Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm.

Biological Context

Subcellular Location: Endoplasmic reticulum membrane (Single-pass membrane protein); Cell membrane (Single-pass type I membrane protein)
Tissue Specificity: Expressed only in mature pollen, in the two sperm cells

Product Specifications

Recombinant Arabidopsis thaliana Protein HAPLESS 2 (HAP2) Protein is a recombinant protein from Arabidopsis thaliana (Mouse-ear cress), expressed in in vitro E.coli expression system, covering amino acids 25-705aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 80.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Required for male fertility (PubMed:17079265, PubMed:20333238). Plays a role in pollen tube guidance and successful gamete attachment (PubMed:17079265). Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm (PubMed:17079265, PubMed:20333238, PubMed:21209845). Mediates the fusion of cell membranes (PubMed:28137780). Not required for pollen tube outgrowth (PubMed:17079265, PubMed:20333238)

Subcellular Location

Endoplasmic reticulum membrane (Single-pass membrane protein); Cell membrane (Single-pass type I membrane protein)

Tissue Specificity

Expressed only in mature pollen, in the two sperm cells

Gene: HAP2  |  Organism: Arabidopsis thaliana  |  Synonyms: GENERATIVE CELL SPECIFIC 1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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