Recombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag), partial, Avian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A)), E.coli, N-terminal GST-tagged, 52.2kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag), partial

Recombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag), partial — Aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Purity >90%.

SKU: BCRECP-002807 Categories: , ,
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Product Specifications

Product SkuBCRECP-002807
Product DescriptionRecombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 240-476aa. [Accession | P0C776].
Uniprot No.P0C776
Gene Namesgag
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region240-476aa
SpeciesAvian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A))
Tag InfoN-terminal GST-tagged
Molecular weight52.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation.

Biological Context

Subcellular Location: Host nucleus, host nucleolus; Host nucleus, host nucleoplasm

Product Specifications

Recombinant Avian leukosis virus RSA Gag-Pro polyprotein (gag), partial is a recombinant protein from Avian leukosis virus RSA (RSV-SRA) (Rous sarcoma virus (strain Schmidt-Ruppin A)), expressed in E.coli, covering amino acids 240-476aa, with N-terminal GST-tagged tag, molecular weight 52.2kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Aspartyl protease that mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell

Subcellular Location

Host nucleus, host nucleolus; Host nucleus, host nucleoplasm

Subunit

Homohexamer. Interacts (via p2B domain) with host PACSIN2; this interaction probably allows PACSIN2 recruitment to viral assembly sites

Gene: gag  |  Organism: Avian leukosis virus subgroup A (isolate RSA)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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