Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial, Moloney murine leukemia virus (isolate Shinnick) (MoMLV), E.coli, N-terminal GST-tagged, 57.6kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial

Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial — Involved in the packaging and encapsidation of two copies of the genome. Purity >90%.

SKU: BCRECP-002810 Categories: ,
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Product Specifications

Product SkuBCRECP-002810
Product DescriptionRecombinant Moloney murine leukemia virus Gag polyprotein (gag) Protein is expressed from E.coli with N-terminal GST-tagged. It contains 216-478aa. [Accession | P03332].
Uniprot No.P03332
Gene Namesgag
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region216-478aa
SpeciesMoloney murine leukemia virus (isolate Shinnick) (MoMLV)
Tag InfoN-terminal GST-tagged
Molecular weight57.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.

Biological Context

Subcellular Location: Host cytoplasm

Product Specifications

Recombinant Moloney murine leukemia virus Gag polyprotein (gag), partial is a recombinant protein from Moloney murine leukemia virus (isolate Shinnick) (MoMLV), expressed in E.coli, covering amino acids 216-478aa, with N-terminal GST-tagged tag, molecular weight 57.6kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization

Subcellular Location

Host cytoplasm

Subunit

Interacts (via PPXY motif) with host NEDD4 (PubMed:15908698). Interacts (via PSAP motif) with host TSG101 (PubMed:15908698). Interacts (via LYPX(n)L motif) with host PDCD6IP (PubMed:15908698)

Gene: gag  |  Organism: Moloney murine leukemia virus (isolate Shinnick)  |  Synonyms: Core polyprotein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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