Recombinant Bacillus anthracis Protective antigen (pagA), Bacillus anthracis, E.coli, N-terminal 6xHis-tagged, 89.6kDa, purity >95% as determined by SDS-PAGE.

Recombinant Bacillus anthracis Protective antigen (pagA)

Recombinant Bacillus anthracis Protective antigen (pagA) — Mature form that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol. Purity >95%.

SKU: BCRECP-004007 Category:
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Product Specifications

Product SkuBCRECP-004007
Product DescriptionRecombinant Bacillus anthracis Protective antigen (pagA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 30-764aa. [Accession | P13423].
Uniprot No.P13423
Gene NamespagA
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region30-764aa
SpeciesBacillus anthracis
Tag InfoN-terminal 6xHis-tagged
Molecular weight89.6kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Mature form that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol. Following attachment to host cell receptors and cleavage by FURIN, homooligomerizes to form ring-shaped oligomers that are in a pre-pore conformation, and associates with EF and LF. Toxin-leaded complexes are then endocytosed in a clathrin-dependent process, followed by a conformational change of oligomerized PA-63 from the pre-pore to pore state, which is triggered by the low pH in the endosome.

Biological Context

Subcellular Location: Host cell membrane (Multi-pass membrane protein); Host endosome membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Bacillus anthracis Protective antigen (pagA) is a recombinant protein from Bacillus anthracis, expressed in E.coli, covering amino acids 30-764aa, with N-terminal 6xHis-tagged tag, molecular weight 89.6kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Mature form that oligomerizes and forms a pore to translocate the enzymatic toxin components edema factor (EF) and lethal factor (LF) into the host cytosol (PubMed:15243628, PubMed:15326297). Following attachment to host cell receptors and cleavage by FURIN, homooligomerizes to form ring-shaped oligomers that are in a pre-pore conformation, and associates with EF and LF (PubMed:10085027, PubMed:12117959, PubMed:15313199). Toxin-leaded complexes are then endocytosed in a clathrin-dependent process, followed by a conformational change of oligomerized PA-63 from the pre-pore to pore state, which is triggered by the low pH in the endosome (PubMed:10085027, PubMed:12551953, PubMed:15326297, PubMed:20221438). Once active, the pore mediates unfolding of EF and LF, which pass through the pore and translocate into the host cytosol (PubMed:16051798, PubMed:21037566, PubMed:32047164, PubMed:32521227, PubMed:32810181)

Subcellular Location

Host cell membrane (Multi-pass membrane protein); Host endosome membrane (Multi-pass membrane protein)

Subunit

Homooligomer; homooligomerizes to form homoheptamers (PA-63(7)) or homooctamers (PA-63(8)) (PubMed:10085027, PubMed:16051798, PubMed:19627991, PubMed:20433851, PubMed:25778700, PubMed:32810181). PA-63(7) or PA-63(8) form ring-shaped oligomers that are in a pre-pore conformation, which do not penetrate the host membrane (PubMed:19627991, PubMed:20433851, PubMed:32810181). PA-63(8) displays an enhanced stability, suggesting that this form circulates in the blood to reach and exert toxicity even in distant tissues (PubMed:20433851). Interacts with lethal factor (LF) and edema factor (EF); can bind LF and EF simultaneously and interaction takes place following homooligomerization on the host cell membrane (PubMed:10085027, PubMed:12117959, PubMed:15313199, PubMed:21037566, PubMed:32047164, PubMed:32521227, PubMed:32810181). PA-63(7) homoheptamer interacts with three molecules of LF to form the PA(7)LF(3) complex, in which the relative position of the N-terminal alpha-helices in the three LFs determines which factor is translocated first (PubMed:32810181)

Gene: pagA  |  Organism: Bacillus anthracis  |  Synonyms: Anthrax toxins translocating protein; PA-83
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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