Recombinant Bacillus subtilis DNA polymerase I (polA), partial — recombinant protein from Bacillus subtilis (strain 168), expressed in E.coli. Purity >90%.
| Product Sku | BCRECP-004136 |
| Product Description | Recombinant Bacillus subtilis DNA polymerase I (polA) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 297-880aa. [Accession | O34996]. |
| Uniprot No. | O34996 |
| Gene Names | polA |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Expression System | E.coli |
| Expression Region | 297-880aa |
| Species | Bacillus subtilis (strain 168) |
| Tag Info | C-terminal 6xHis-tagged |
| Molecular weight | 72.8kDa |
| Activity | Please contact us to obtain bioactivity data. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose. |
| Storage | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Research Areas | Others |
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity.
Recombinant Bacillus subtilis DNA polymerase I (polA), partial is a recombinant protein from Bacillus subtilis (strain 168), expressed in E.coli, covering amino acids 297-880aa, with C-terminal 6xHis-tagged tag, molecular weight 72.8kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.
Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.
Biological Activity: Please contact us to obtain bioactivity data.
Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity
Single-chain monomer with multiple functions
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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.
Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.
Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.
Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.
