Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial, Bothrops jararaca (Jararaca) (Bothrops jajaraca), E.coli, N-terminal 6xHis-KSI-tagged, 18.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial

Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial — recombinant protein from Bothrops jararaca (Jararaca) (Bothrops jajaraca), expressed in E.coli. Purity >85%.

SKU: BCRECP-003611 Category:
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Product Specifications

Product SkuBCRECP-003611
Product DescriptionRecombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial Protein is expressed from E.coli with N-terminal 6xHis-KSI-tagged. It contains 1-25aa. [Accession | P22028].
Uniprot No.P22028
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-25aa
SpeciesBothrops jararaca (Jararaca) (Bothrops jajaraca)
Tag InfoN-terminal 6xHis-KSI-tagged
Molecular weight18.0kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research Areasothers

Function

Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation.

Biological Context

Subcellular Location: Secreted
Tissue Specificity: Expressed by the venom gland

Product Specifications

Recombinant Bothrops jararaca Zinc metalloproteinase-disintegrin-like botrocetin, partial is a recombinant protein from Bothrops jararaca (Jararaca) (Bothrops jajaraca), expressed in E.coli, covering amino acids 1-25aa, with N-terminal 6xHis-KSI-tagged tag, molecular weight 18.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Metalloproteinase that binds to von Willebrand factor (VWF) and induces its interaction with GPIb (GP1BA), resulting in platelet aggregation

Subcellular Location

Secreted

Tissue Specificity

Expressed by the venom gland

Subunit

Monomer. Botrocetin and vWF form a soluble complex

Organism: Bothrops jararaca  |  Synonyms: Snake venom metalloproteinase; von Willebrand factor-dependent platelet coagglutinin

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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