Recombinant Bovine Alpha-crystallin B chain (CRYAB), Bos taurus (Bovine), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 27.5kDa, purity >90% as determined by SDS-PAGE.

Recombinant Bovine Alpha-crystallin B chain (CRYAB)

Recombinant Bovine Alpha-crystallin B chain (CRYAB) — May contribute to the transparency and refractive index of the lens. Purity >90%.

SKU: BCRECP-002447 Category:
Online Inquiry →

Product Specifications

Product SkuBCRECP-002447
Product DescriptionRecombinant Bovine Alpha-crystallin B chain (CRYAB) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-175aa. [Accession | P02510].
Uniprot No.P02510
Gene NamesCRYAB
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region1-175aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight27.5kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Secreted; Lysosome
Tissue Specificity: Lens as well as other tissues

Product Specifications

Recombinant Bovine Alpha-crystallin B chain (CRYAB) is a recombinant protein from Bos taurus (Bovine), expressed in E.coli, covering amino acids 1-175aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 27.5kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity)

Subcellular Location

Cytoplasm; Nucleus; Secreted; Lysosome

Tissue Specificity

Lens as well as other tissues

Subunit

Heteromer composed of three CRYAA and one CRYAB subunits. Aggregates with homologous proteins, including the small heat shock protein HSPB1, to form large heteromeric complexes. Inter-subunit bridging via zinc ions enhances stability, which is crucial as there is no protein turn over in the lens. Interacts with HSPBAP1 and TTN/titin. Interacts with TMEM109; in the cellular response to DNA damage. Interacts with DES; binds rapidly during early stages of DES filament assembly and a reduced binding seen in the later stages. Interacts with TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion. Interacts with ATP6V1A and with MTOR, forming a ternary complex (By similarity)

Gene: CRYAB  |  Organism: Bos taurus  |  Synonyms: Alpha(B)-crystallin
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top