Recombinant Bovine Rhodopsin (RHO) Protein, Bos taurus (Bovine), in vitro E.coli expression system, N-terminal 6xHis-tagged, 45.0kDa, purity >85% as determined by SDS-PAGE.

Recombinant Bovine Rhodopsin (RHO) Protein

Recombinant Bovine Rhodopsin (RHO) Protein — G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions. Purity >85%.

SKU: BCRECP-00313TM Category:
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Product Specifications

Uniprot No.P02699
Gene NamesRHO
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-348aa
SpeciesBos taurus (Bovine)
Tag InfoN-terminal 6xHis-tagged
Molecular weight45.0kDa
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions. Required for postnatal photoreceptor cell viability. Activation occurs when the covalently bound 11-cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers G protein-mediated phototransduction (Probable).

Biological Context

Subcellular Location: Photoreceptor outer segment membrane (Multi-pass membrane protein); Membrane (Multi-pass membrane protein)
Tissue Specificity: Expressed in rod-shaped photoreceptor cells in the retina that mediate vision in dim light (at protein level)

Product Specifications

Recombinant Bovine Rhodopsin (RHO) Protein is a recombinant protein from Bos taurus (Bovine), expressed in in vitro E.coli expression system, covering amino acids 1-348aa, with N-terminal 6xHis-tagged tag, molecular weight 45.0kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Not implied unless activity data are explicitly listed on this product page, the datasheet, or the CoA.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

G protein-coupled photoreceptor that activates the G protein transducin (t) signaling pathway in response to light and is essential for image-forming vision under low-light conditions (PubMed:31300275). Required for postnatal photoreceptor cell viability (By similarity). Activation occurs when the covalently bound 11-cis-retinal chromophore absorbs a photon and isomerizes to all-trans-retinal, inducing a conformational change in the opsin that triggers G protein-mediated phototransduction (Probable) (PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:31300275). Signal termination occurs via receptor phosphorylation, which promotes binding of arrestin (SAG) and displacement of the G protein alpha subunit (Probable) (PubMed:1396673)

Subcellular Location

Photoreceptor outer segment membrane (Multi-pass membrane protein); Membrane (Multi-pass membrane protein)

Tissue Specificity

Expressed in rod-shaped photoreceptor cells in the retina that mediate vision in dim light (at protein level)

Subunit

Homodimer (PubMed:18563085, PubMed:23303210). May form a complex composed of RHO, GRK1 and RCVRN in a Ca(2+)-dependent manner; RCVRN prevents the interaction between GRK1 and RHO (PubMed:17020884). Interacts with GRK1 (By similarity). Interacts (phosphorylated form) with SAG (PubMed:15111114, PubMed:15351781, PubMed:23579341, PubMed:25205354, PubMed:26200343). Interacts with GNAT1 (PubMed:18818650, PubMed:21389983, PubMed:23303210, PubMed:23579341, PubMed:26526852, PubMed:28655769, PubMed:31300275). Interacts with GNAT3 (PubMed:22198838, PubMed:27458239). SAG and G proteins compete for a common binding site (By similarity). Interacts with PRCD; the interaction promotes PRCD stability (PubMed:27509380). Forms a complex with ASAP1 and ARF4 (PubMed:25673879). Forms a complex with ASAP1, RAB11A, Rabin8/RAB3IP, ARF4 and RAB11FIP3; the complex regulates Golgi-to-cilia rhodopsin/RHO transport in photoreceptors (PubMed:25673879)

Gene: RHO  |  Organism: Bos taurus
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

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Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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