Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1), Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast), Yeast, N-terminal 6xHis-tagged, 33.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1)

Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1) — Cell surface protein involved in the host-parasite interaction during candidal infection. Purity >90%.

SKU: BCRECP-004152 Category:
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Product Specifications

Product SkuBCRECP-004152
Product DescriptionRecombinant Candida albicans pH-regulated antigen PRA1 (PRA1) Protein is expressed from Yeast with N-terminal 6xHis-tagged. It contains 16-299aa. [Accession | P87020].
Uniprot No.P87020
Gene NamesPRA1
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region16-299aa
SpeciesCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Tag InfoN-terminal 6xHis-tagged
Molecular weight33.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Cell surface protein involved in the host-parasite interaction during candidal infection. With MP65, represents a major component of the biofilm matrix. As a surface protein, binds the two human complement regulators CFH and CFHR1, as well as plasminogen PLG, mediates complement evasion and extra-cellular matrix interaction and/or degradation.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Candida albicans pH-regulated antigen PRA1 (PRA1) is a recombinant protein from Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast), expressed in Yeast, covering amino acids 16-299aa, with N-terminal 6xHis-tagged tag, molecular weight 33.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Cell surface protein involved in the host-parasite interaction during candidal infection (PubMed:17277107, PubMed:19850343, PubMed:20504767, PubMed:20644161, PubMed:21212281, PubMed:21245270, PubMed:21820180, PubMed:22074954, PubMed:22761575, PubMed:22844116, PubMed:9440517). With MP65, represents a major component of the biofilm matrix (PubMed:17277107). As a surface protein, binds the two human complement regulators CFH and CFHR1, as well as plasminogen PLG, mediates complement evasion and extra-cellular matrix interaction and/or degradation (PubMed:19850343, PubMed:20504767). As a released protein, enhances complement control in direct vicinity of the yeast and thus generates an additional protective layer which controls host complement attack, assisting the fungus in escaping host surveillance (PubMed:17277107, PubMed:19850343). Binds to host fluid-phase C3 and blocks cleavage of C3 to C3a and C3b, leading to inhibition of complement activation and protection from uptake of C.albicans by human macrophages (PubMed:20644161). Also mediates human complement control and complement evasion through binding to C4BPA, another human complement inhibitor, as well as through binding to host integrin alpha-M/beta-2 (PubMed:21212281, PubMed:21245270, PubMed:21820180, PubMed:22844116). Binds zinc from its environment and then reassociates with ZRT1 to acquire this essential metal (PubMed:22761575)

Subcellular Location

Secreted

Subunit

Component of a multiprotein complex of 250 kDa composed of at least HYR1, MP65, and PRA1 (PubMed:17277107). Interacts with host Integrin alpha-M/beta-2 heterodimer (PubMed:17277107). Also binds human factor H (CFH), CFHR1, plasminogen (PLG), complement C3, and C4BPA (PubMed:19850343, PubMed:20644161, PubMed:21212281, PubMed:21245270). Interacts with ZRT101 (PubMed:22761575)

Gene: PRA1  |  Organism: Candida albicans (strain SC5314 / ATCC MYA-2876)  |  Synonyms: 58 kDa fibrinogen-binding mannoprotein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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