Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated, Gallus gallus (Chicken), E.coli, N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged, 83.3kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated

Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated — Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Purity >90%.

SKU: BCRECP-002818 Categories: , , ,
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Product Specifications

Product SkuBCRECP-002818
Product DescriptionRecombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Protein is expressed from E.coli with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. It contains 2-333aa. [Accession | P00356].
Uniprot No.P00356
Gene NamesGAPDH
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-333aa
SpeciesGallus gallus (Chicken)
Tag InfoN-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged
Molecular weight83.3kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Product Specifications

Recombinant Chicken Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), partial, Biotinylated is a recombinant protein from Gallus gallus (Chicken), expressed in E.coli, covering amino acids 2-333aa, with N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged tag, molecular weight 83.3kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)

Subcellular Location

Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus

Subunit

Homotetramer

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Gene: GAPDH  |  Organism: Gallus gallus  |  Synonyms: Peptidyl-cysteine S-nitrosylase GAPDH
Key Publications

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How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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