Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus), E.coli, N-terminal 6xHis-tagged, 39.7kDa, purity >85% as determined by SDS-PAGE.
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Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)

Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) — Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Purity >85%.

SKU: BCRECP-002819 Categories: ,
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Product Specifications

Product SkuBCRECP-002819
Product DescriptionRecombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2-333aa. [Accession | P17244].
Uniprot No.P17244
Gene NamesGAPDH
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2-333aa
SpeciesCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Tag InfoN-terminal 6xHis-tagged
Molecular weight39.7kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasCancer

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton.

Biological Context

Subcellular Location: Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus
Pathway: Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Product Specifications

Recombinant Cricetulus griseus Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a recombinant protein from Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus), expressed in E.coli, covering amino acids 2-333aa, with N-terminal 6xHis-tagged tag, molecular weight 39.7kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions (By similarity). Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity)

Subcellular Location

Cytoplasm, cytosol; Cytoplasm, cytoskeleton; Nucleus

Subunit

Homotetramer (By similarity). Interacts with TPPP; the interaction is direct (By similarity). Interacts (when S-nitrosylated) with SIAH1; leading to nuclear translocation. Interacts with RILPL1/GOSPEL, leading to prevent the interaction between GAPDH and SIAH1 and prevent nuclear translocation. Interacts with CHP1; the interaction increases the binding of CHP1 with microtubules. Associates with microtubules (By similarity). Interacts with EIF1AD, USP25, PRKCI and WARS1. Interacts with phosphorylated RPL13A; inhibited by oxidatively-modified low-densitity lipoprotein (LDL(ox)). Component of the GAIT complex. Interacts with FKBP6; leading to inhibit GAPDH catalytic activity. Interacts with TRAF2, promoting TRAF2 ubiquitination. Interacts with TRAF3, promoting TRAF3 ubiquitination (By similarity)

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5

Gene: GAPDH  |  Organism: Cricetulus griseus  |  Synonyms: Peptidyl-cysteine S-nitrosylase GAPDH

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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