Recombinant Clostridioides difficile Toxin A (toxA), partial, Clostridioides difficile (Peptoclostridium difficile), E.coli, N-terminal 6xHis-tagged, 40.1kDa, purity >85% as determined by SDS-PAGE.

Recombinant Clostridioides difficile Toxin A (toxA), partial

Recombinant Clostridioides difficile Toxin A (toxA), partial — Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases. Purity >85%.

SKU: BCRECP-004594 Category:
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Product Specifications

Product SkuBCRECP-004594
Product DescriptionRecombinant Clostridioides difficile Toxin A (toxA) Protein is expressed from E.coli with N-terminal 6xHis-tagged. It contains 2387-2710aa. [Accession | P16154].
Uniprot No.P16154
Gene NamestoxA
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region2387-2710aa
SpeciesClostridioides difficile (Peptoclostridium difficile)
Tag InfoN-terminal 6xHis-tagged
Molecular weight40.1kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasMicrobiology

Function

Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases. Acts by mediating monoglucosylation of small GTPases of the Rho family (Rac1, RhoA, RhoB, RhoC, Rap2A and Cdc42) in host cells at the conserved threonine residue located in the switch I region ('Thr-37/35'), using UDP-alpha-D-glucose as the sugar donor. Monoglucosylation of host small GTPases completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function.

Biological Context

Subcellular Location: Host cytoplasm, host cytosol; Host cell membrane (Peripheral membrane protein)

Product Specifications

Recombinant Clostridioides difficile Toxin A (toxA), partial is a recombinant protein from Clostridioides difficile (Peptoclostridium difficile), expressed in E.coli, covering amino acids 2387-2710aa, with N-terminal 6xHis-tagged tag, molecular weight 40.1kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Please contact us to obtain bioactivity data.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Active form of the toxin, which is released into the host cytosol following autoprocessing and inactivates small GTPases (PubMed:22267739, PubMed:22747490, PubMed:24905543, PubMed:30622517, PubMed:7775453). Acts by mediating monoglucosylation of small GTPases of the Rho family (Rac1, RhoA, RhoB, RhoC, Rap2A and Cdc42) in host cells at the conserved threonine residue located in the switch I region ('Thr-37/35'), using UDP-alpha-D-glucose as the sugar donor (PubMed:22267739, PubMed:22747490, PubMed:24905543, PubMed:30622517, PubMed:7775453). Monoglucosylation of host small GTPases completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption and cell death, resulting in the loss of colonic epithelial barrier function (PubMed:7775453). Also able to catalyze monoglucosylation of some members of the Ras family (H-Ras/HRAS, K-Ras/KRAS and N-Ras/NRAS), but with much less efficiency than with Rho proteins, suggesting that it does not act on Ras proteins in vivo (PubMed:30622517)

Subcellular Location

Host cytoplasm, host cytosol; Host cell membrane (Peripheral membrane protein)

Subunit

Interacts with host LDLR; LDLR probably does not constitute a major receptor but may contribute to entry into cells

Gene: tcdA  |  Organism: Clostridioides difficile
Key Publications

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Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

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Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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