Recombinant Clostridium botulinum C phage Hemagglutinin components HA-70 type C (HA-70), partial, Clostridium botulinum, E.coli, C-terminal 6xHis-tagged, 28.4kDa, purity >90% as determined by SDS-PAGE.

Recombinant Clostridium botulinum C phage Hemagglutinin components HA-70 type C (HA-70), partial

Recombinant Clostridium botulinum C phage Hemagglutinin components HA-70 type C (HA-70), partial — The hemagglutinin (HA) component of the progenitor toxin protects the structural integrity of botulinum neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host.

SKU: BCRECP-002964 Category:
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Product Specifications

Product SkuBCRECP-002964
Product DescriptionRecombinant Clostridium botulinum C phage Hemagglutinin components HA-70 type C (HA-70) Protein is expressed from E.coli with C-terminal 6xHis-tagged. It contains 7-192aa. [Accession | P46085].
Uniprot No.P46085
Gene NamesHA-70
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region7-192aa
SpeciesClostridium botulinum
Tag InfoC-terminal 6xHis-tagged
Molecular weight28.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

The hemagglutinin (HA) component of the progenitor toxin protects the structural integrity of botulinum neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host. The HA component is involved in binding to the upper small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties (Probable). Whole protein and the HA-53 chain (but not the HA-22-23 chain) bind to bovine mucin; if the mucin is pretreated with neuraminidase (removes the terminal sialic acid of glycoconjugates) mucin binding is decreased.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Clostridium botulinum C phage Hemagglutinin components HA-70 type C (HA-70), partial is a recombinant protein from Clostridium botulinum, expressed in E.coli, covering amino acids 7-192aa, with C-terminal 6xHis-tagged tag, molecular weight 28.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

The hemagglutinin (HA) component of the progenitor toxin protects the structural integrity of botulinum neurotoxin; may increase internalization of the neurotoxin into the bloodstream of the host (PubMed:9421908). The HA component is involved in binding to the upper small intestine through interactions with glycolipids and glycoproteins containing sialic acid moieties (Probable). Whole protein and the HA-53 chain (but not the HA-22-23 chain) bind to bovine mucin; if the mucin is pretreated with neuraminidase (removes the terminal sialic acid of glycoconjugates) mucin binding is decreased (PubMed:19071137). Has higher affinity for alpha-2,3-sialylated oligosaccharides than alpha-2-6 sialylated oligosaccharides (PubMed:22684008)

Subcellular Location

Secreted

Subunit

Botulinum toxins are produced as progenitor toxins of large molecular sizes of 12S (M toxin) and 16S (L toxin). M toxin consists of a non-toxic, non-hemagglutinin component (NTNHA) and the neurotoxin (Probable). L toxin consists of the M toxin and the 3 subcomponents of hemagglutinin (HA) (PubMed:7802661). HA is composed of subcomponents of 70, 33, and 17 kDa (PubMed:7802661). The 70 kDa subcomponent undergoes proteolytic processing and is split into HA-53 (also called HA3b) and HA-22-23 (collectively called HA3a) (PubMed:7802661). Upon crystallization this cleaved component forms a three-lobed trimer of dimers with a hole in the center (PubMed:19071137, PubMed:22684008). Preliminary crystallization studies show a tri-lobed HA-70 where each HA-70 contacts one HA-17 molecule (Probable). The stoichiometry of the whole complex has been modeled as one BoNT/C, one NTNHA, three HA-70, six HA-33 and three HA-17 (By similarity)

Gene: HA-70  |  Organism: Clostridium botulinum C phage  |  Synonyms: HA3; Hemagglutinin components HA-22/23/53 type C; Hemagglutinin components HA-22/23/53 type C1
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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