Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active), Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV), Mammalian cell, C-terminal 10xHis-tagged, 42.2 kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active)

Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active) — Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes. Purity >95%.

SKU: BCACP-000354 Categories: ,
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Product SKUBCACP-000354
Product DescriptionRecombinant CCHFV Envelopment polyprotein (GP) protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1078-1439aa. [Accession | Q8JSZ3].
Uniprot No.Q8JSZ3
Gene NamesGP
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1078-1439aa
SpeciesCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Tag InfoC-terminal 10xHis-tagged
Molecular weight42.2 kDa
ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Human SIRPA protein. The EC50 is 98.73-112.7 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Anti-CD47 recombinant antibody . The EC50 is 1.343-1.561 ng/mL.
BufferLyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Alternative NamesEnvelopment polyprotein; M polyprotein; Mucin-like variable region; GP38; Glycoprotein NNon-Structural protein M; Glycoprotein C; GP

Function

Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes. Attachment to receptor induces virion internalization predominantly through clathrin-dependent endocytosis. Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion.

Biological Context

Subcellular Location: Host Golgi apparatus membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active) is a recombinant protein from Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV), expressed in Mammalian cell, covering amino acids 1078-1439aa, with C-terminal 10xHis-tagged tag, molecular weight 42.2 kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

Recommended applications depend on the target, product format, and product-specific validation data. Refer to the product datasheet or Certificate of Analysis (CoA), or contact BioCrest Sci for application guidance.

Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Human SIRPA protein. The EC50 is 98.73-112.7 ng/mL.;Measured by its binding ability in a functional ELISA. Immobilized Human CD47 at 2 μg/mL can bind Anti-CD47 recombinant antibody . The EC50 is 1.343-1.561 ng/mL.

Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shipping & Lead Time: Availability, shipping conditions, and estimated lead time are confirmed in the formal quotation or order confirmation based on the product format, destination, and order requirements.

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Protein Biology

Function

Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes (PubMed:38182887). Attachment to receptor induces virion internalization predominantly through clathrin-dependent endocytosis (PubMed:19088291). Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion (PubMed:34793197, PubMed:35234630). Exposure of the glycoprotein spikes to potassium is necessary for the conformational change leading to fusion (By similarity)

Subcellular Location

Host Golgi apparatus membrane (Multi-pass membrane protein)

Subunit

Heterodimer with glycoprotein N; in prefusion state (PubMed:34793197). Homotrimeric; in postfusion state (PubMed:34793197, PubMed:35234630). Interacts with host LDLR protein (PubMed:38182887)

Gene: GP  |  Organism: Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970)  |  Synonyms: M polyprotein
Key Publications

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Frequently Asked Questions

How do I order or inquire about this product?

Submit the Online Inquiry form with your required quantity and specifications, or email sales@biocrestsci.com. Available sizes, pricing, stock status, documentation, and estimated lead time are confirmed in the formal quotation.

What is the shipping and delivery time?

Availability, dispatch timing, shipping conditions, and delivery estimates depend on the product format, destination, and order requirements. These details are confirmed in the formal quotation or order confirmation.

How should this product be stored?

Follow the storage and handling instructions in the product-specific datasheet and Certificate of Analysis (CoA). Requirements may vary by product form and formulation; contact BioCrest Sci if product-specific guidance is needed.

What quality controls are performed on your products?

Quality-control data such as identity, purity, endotoxin level, or biological activity are provided only when specified on this product page, the datasheet, or Certificate of Analysis (CoA). Biological activity is not implied unless explicitly stated.

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