Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active), Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV), Mammalian cell, C-terminal 10xHis-tagged, 42.2kDa, purity >95% as determined by SDS-PAGE.
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Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active)

Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active) — Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes. Purity >95%.

SKU: BCRECP-002889 Categories: ,
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Product Specifications

Product SkuBCRECP-002889
Product DescriptionRecombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP) Protein is expressed from Mammalian cell with C-terminal 10xHis-tagged. It contains 1078-1439aa. [Accession | Q8JSZ3].
Uniprot No.Q8JSZ3
Gene NamesGP
PurityGreater than 95% as determined by SDS-PAGE.
Expression SystemMammalian cell
Expression Region1078-1439aa
SpeciesCrimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV)
Tag InfoC-terminal 10xHis-tagged
Molecular weight42.2kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasSignal Transduction

Function

Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes. Attachment to receptor induces virion internalization predominantly through clathrin-dependent endocytosis. Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion.

Biological Context

Subcellular Location: Host Golgi apparatus membrane (Multi-pass membrane protein)

Product Specifications

Recombinant Crimean-Congo hemorrhagic fever virus Envelopment polyprotein (GP), partial (Active) is a recombinant protein from Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970) (CCHFV), expressed in Mammalian cell, covering amino acids 1078-1439aa, with C-terminal 10xHis-tagged tag, molecular weight 42.2kDa, purity Greater than 95% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Binds to host cell surface receptor LDLR and mediates fusion between viral and cellular membranes (PubMed:38182887). Attachment to receptor induces virion internalization predominantly through clathrin-dependent endocytosis (PubMed:19088291). Class II fusion protein that promotes fusion of viral membrane with host endosomal membrane after endocytosis of the virion (PubMed:34793197, PubMed:35234630). Exposure of the glycoprotein spikes to potassium is necessary for the conformational change leading to fusion (By similarity)

Subcellular Location

Host Golgi apparatus membrane (Multi-pass membrane protein)

Subunit

Heterodimer with glycoprotein N; in prefusion state (PubMed:34793197). Homotrimeric; in postfusion state (PubMed:34793197, PubMed:35234630). Interacts with host LDLR protein (PubMed:38182887)

Gene: GP  |  Organism: Crimean-Congo hemorrhagic fever virus (strain Nigeria/IbAr10200/1970)  |  Synonyms: M polyprotein
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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