Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5), Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans), Yeast, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 28.4kDa, purity >90% as determined by SDS-PAGE.
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Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5)

Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) — recombinant protein from Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans), expressed in Yeast. Purity >90%.

SKU: BCRECP-004688 Categories: ,
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Product Specifications

Product SkuBCRECP-004688
Product DescriptionRecombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) Protein is expressed from Yeast with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 1-225aa. [Accession | P0CS95].
Uniprot No.P0CS95
Gene NamesURA5
PurityGreater than 90% as determined by SDS-PAGE.
Expression SystemYeast
Expression Region1-225aa
SpeciesCryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight28.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).

Biological Context

Pathway: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2

Product Specifications

Recombinant Cryptococcus neoformans var. neoformans serotype D Orotate phosphoribosyltransferase (URA5) is a recombinant protein from Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) (Filobasidiella neoformans), expressed in Yeast, covering amino acids 1-225aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 28.4kDa, purity Greater than 90% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

Subunit

Homodimer

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2

Gene: URA5  |  Organism: Cryptococcus deneoformans (strain JEC21 / ATCC MYA-565)

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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