Recombinant Dog CD40 ligand (CD40LG) Protein, Canis lupus familiaris (Dog) (Canis familiaris), in vitro E.coli expression system, N-terminal 10xHis-tagged, 30.2kDa, purity >85% as determined by SDS-PAGE.
,

Recombinant Dog CD40 ligand (CD40LG) Protein

Recombinant Dog CD40 ligand (CD40LG) Protein — recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in in vitro E.coli expression system. Purity >85%.

SKU: BCRECP-00244TM Categories: ,
Online Inquiry →

Product Specifications

Uniprot No.O97626
Gene NamesCD40LG
PurityGreater than 85% as determined by SDS-PAGE.
Expression Systemin vitro E.coli expression system
Expression Region1-260aa
SpeciesCanis lupus familiaris (Dog) (Canis familiaris)
Tag InfoN-terminal 10xHis-tagged
Molecular weight30.2kDa
ActivityNot Test
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.

Biological Context

Subcellular Location: Secreted

Product Specifications

Recombinant Dog CD40 ligand (CD40LG) Protein is a recombinant protein from Canis lupus familiaris (Dog) (Canis familiaris), expressed in in vitro E.coli expression system, covering amino acids 1-260aa, with N-terminal 10xHis-tagged tag, molecular weight 30.2kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

Related products

Protein Biology

Function

Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling

Subcellular Location

Secreted

Subunit

Homotrimer (By similarity). Interacts with CD28 (By similarity). CD40 ligand, soluble form: Exists as either a monomer or a homotrimer (By similarity). Forms a ternary complex between CD40 and integrins for CD40-CD40LG signaling (By similarity)

Gene: CD40LG  |  Organism: Canis lupus familiaris  |  Synonyms: Tumor necrosis factor ligand superfamily member 5

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

Shopping Cart
Scroll to Top