Recombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2), partial, Drosophila melanogaster (Fruit fly), E.coli, N-terminal 10xHis-tagged and C-terminal Myc-tagged, 38.4kDa, purity >85% as determined by SDS-PAGE.

Recombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2), partial

Recombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2), partial — May be involved in the export of mRNA from the nucleus to the cytoplasm. Purity >85%.

SKU: BCRECP-003921 Category:
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Product Specifications

Product SkuBCRECP-003921
Product DescriptionRecombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2) Protein is expressed from E.coli with N-terminal 10xHis-tagged and C-terminal Myc-tagged. It contains 573-841aa. [Accession | Q9VV73].
Uniprot No.Q9VV73
Gene Namesnxf2
PurityGreater than 85% as determined by SDS-PAGE.
Expression SystemE.coli
Expression Region573-841aa
SpeciesDrosophila melanogaster (Fruit fly)
Tag InfoN-terminal 10xHis-tagged and C-terminal Myc-tagged
Molecular weight38.4kDa
ActivityPlease contact us to obtain bioactivity data.
BufferIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
StorageStore at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Research AreasOthers

Function

May be involved in the export of mRNA from the nucleus to the cytoplasm. In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing. On recruitment to a target transcript, interacts with single stranded RNA, thereby anchoring the complex via the nascent target transcript to chromatin and allowing Panx to recruit silencing effectors to establishing repressive heterochromatin at transposon loci.

Biological Context

Subcellular Location: Cytoplasm; Nucleus; Nucleus, nucleoplasm
Tissue Specificity: Expressed in female gonads (at protein level). Expressed ubiquitously

Product Specifications

Recombinant Drosophila melanogaster Nuclear RNA export factor 2 (nxf2), partial is a recombinant protein from Drosophila melanogaster (Fruit fly), expressed in E.coli, covering amino acids 573-841aa, with N-terminal 10xHis-tagged and C-terminal Myc-tagged tag, molecular weight 38.4kDa, purity Greater than 85% as determined by SDS-PAGE.. Suitable for ELISA and Western Blot applications.

SDS-PAGE: Single band at expected molecular weight confirming purity.

ELISA: Suitable as coating antigen or detection standard.

Western Blot: Compatible with standard Western Blot protocols.

Protein Interaction: Validated for SPR (Surface Plasmon Resonance) and BLI (Bio-Layer Interferometry) studies.

Shipping: Shipped at ambient temperature. Lyophilized protein is stable during transit.

Storage: Store lyophilized protein at -20°C to -80°C. Reconstituted protein should be aliquoted and stored at -80°C. Avoid repeated freeze-thaw cycles.

Shelf Life: 12 months from date of receipt when stored as recommended.

Shipping Time: Orders placed before 2 PM EST ship same day. International orders typically deliver within 5-10 business days.

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Protein Biology

Function

May be involved in the export of mRNA from the nucleus to the cytoplasm (PubMed:11780633). In the ovaries, forms a complex with nxf2, piwi and Nxt1 which acts as effectors of cotranscriptional transposon silencing (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). On recruitment to a target transcript, interacts with single stranded RNA, thereby anchoring the complex via the nascent target transcript to chromatin and allowing Panx to recruit silencing effectors to establishing repressive heterochromatin at transposon loci (PubMed:31368590, PubMed:31384064, PubMed:31570835). Does not affect piRNA biogenesis (PubMed:31384064). The interaction with Panx stabilizes the nuclear protein complex (PubMed:31384064). Does not bind nucleoporins, but regulates sbr/Nxf1 binding to nucleoporins and, indirectly, transposon exports (PubMed:31384064, PubMed:31570835)

Subcellular Location

Cytoplasm; Nucleus; Nucleus, nucleoplasm

Tissue Specificity

Expressed in female gonads (at protein level) (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). Expressed ubiquitously (PubMed:11780633)

Subunit

In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). The complex is knowns as Panx-induced cotranscriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:31219034, PubMed:31368590, PubMed:31384064, PubMed:31570835). Interacts (via TAP-C domain) with Panx (via NIR region); the interaction is direct (PubMed:31219034, PubMed:31368590, PubMed:31570835). Interacts (via NTF2 domain) with Nxt1; the interaction is direct and prevents Nxt1 binding to nucleoporins (PubMed:31368590). Interacts with sbr/Nxf1 (PubMed:31570835)

Gene: nxf2  |  Organism: Drosophila melanogaster
Key Publications

Frequently Asked Questions

How do I order or inquire about this product?

Fill out the Online Inquiry form with your required quantity and specifications. You can also email sales@biocrestsci.com. Our team typically responds within 4 business hours with a quote and availability confirmation.

What is the shipping and delivery time?

Orders placed before 2 PM EST ship the same day. Domestic (US) delivery typically takes 2-3 business days. International orders deliver within 5-10 business days. All products are shipped at ambient temperature with appropriate packaging to ensure stability.

How should I store this recombinant protein?

Lyophilized proteins should be stored at -20°C to -80°C upon receipt. After reconstitution, aliquot and store at -80°C. Avoid repeated freeze-thaw cycles. Shelf life is 12 months from date of receipt when stored as recommended.

What quality controls are performed on your products?

Each product undergoes SDS-PAGE purity analysis (typically >85-95%), endotoxin testing, and bioactivity validation. Products are validated for ELISA, Western Blot, and SPR/BLI applications as specified on this product page. A Certificate of Analysis (CoA) is available upon request.

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